组织透明化和光片荧光显微镜术对动脉粥样硬化血管的三维可视化。
Three-Dimensional Visualization of Atherosclerotic Vessels by Tissue Clearing and Light-Sheet Fluorescence Microscopy.
机构信息
Laboratory of Molecular Metabolism, The Rockefeller University, New York, NY, USA.
First Department of Medicine (Division of Cardiology), University Medical Center Mannheim, Mannheim, Germany.
出版信息
Methods Mol Biol. 2022;2419:841-851. doi: 10.1007/978-1-0716-1924-7_51.
Although arteries and atherosclerotic plaques are three-dimensional structures, the evaluation of plaque size and morphology in preclinical models of atherosclerosis is typically performed in two dimensions by histological analysis. Here, we describe a method to visualize arteries and atherosclerotic plaques in three dimensions. This method combines AdipoClear, a procedure that achieves whole tissue immunolabeling and clearing, and light-sheet fluorescence microscopy, which generates a three-dimensional reconstruction of vessel architecture including atherosclerotic lesions if present. This approach reveals the volume, geometry, acellular component and surface of atherosclerotic plaques as well as the spatial position of the lesion in relation to the affected artery.
尽管动脉和动脉粥样硬化斑块是三维结构，但在动脉粥样硬化的临床前模型中，斑块大小和形态的评估通常通过组织学分析在二维平面上进行。在这里，我们描述了一种可视化动脉和动脉粥样硬化斑块三维结构的方法。该方法结合了 AdipoClear（一种可实现整个组织免疫标记和清除的程序）和光片荧光显微镜技术，如果存在动脉粥样硬化病变，该技术可生成血管结构的三维重建。这种方法可以显示动脉粥样硬化斑块的体积、几何形状、无细胞成分和表面，以及病变相对于受影响动脉的空间位置。
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