Urena-Saborio Hilary, Alfaro-Viquez Emilia, Esquivel-Alvarado Daniel, Madrigal-Carballo Sergio, Krueger Christian G, Reed Jess D, Gunasekaran Sundaram
Department of Biological Systems Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Reed Research Group, Department of Animal Sciences, University of Wisconsin-Madison, Madison, Wisconsin, USA.
J Biomed Mater Res B Appl Biomater. 2022 Aug;110(8):1876-1886. doi: 10.1002/jbm.b.35045. Epub 2022 Mar 3.
The interaction between A-type interflavan bonds from cranberry proanthocyanidins (PAC) and surface virulence factors of extra-intestinal pathogenic Escherichia coli (ExPEC) was studied. Electrospun nanofibers (ESNF) were fabricated using PAC and polycaprolactone (PCL) solutions and their physical and chemical properties were characterized. The ability of PAC:PCL composite ESNF to interact with and entrap ExPEC strain 5011 (ExPEC-5011) was evaluated in vitro by plate culturing and when formulated as a biofilter and nanocoating. As a biofilter, the PAC:PCL ESNF exhibited a dose-dependent ability to entrap ExPEC-5011. Images from scanning electron and fluorescent microscopies revealed that ESNF sections with higher amounts of PAC led to higher bacterial entrapment. The effectiveness PAC:PCL ESNF to bind ExPEC when applied as a nanocoating was studied using ESNF-coated polyvinyl chloride intermittent catheter. Results indicate that ExPEC-5011 was entrapped well into the PAC:PCL ESNF coating on the catheter. Overall, our results suggest that incorporating the biomolecule PAC in ESNF is a potential means for applications requiring bacterial entrapment, such as biofunctionalization, biofiltration, and surface coating, among others.
研究了蔓越莓原花青素（PAC）中的A型黄酮间键与肠道外致病性大肠杆菌（ExPEC）表面毒力因子之间的相互作用。使用PAC和聚己内酯（PCL）溶液制备了电纺纳米纤维（ESNF），并对其物理和化学性质进行了表征。通过平板培养以及将其制成生物过滤器和纳米涂层，在体外评估了PAC:PCL复合ESNF与ExPEC菌株5011（ExPEC-5011）相互作用并捕获该菌株的能力。作为生物过滤器，PAC:PCL ESNF表现出捕获ExPEC-5011的剂量依赖性能力。扫描电子显微镜和荧光显微镜图像显示，PAC含量较高的ESNF切片导致更高的细菌捕获量。使用涂覆有ESNF的聚氯乙烯间歇导管研究了PAC:PCL ESNF作为纳米涂层应用时结合ExPEC的有效性。结果表明，ExPEC-5011被很好地捕获到导管上的PAC:PCL ESNF涂层中。总体而言，我们的结果表明，在ESNF中掺入生物分子PAC是一种潜在的方法，可用于需要捕获细菌的应用，如生物功能化、生物过滤和表面涂层等。
