单细胞测序揭示再生胰腺中的物理相互作用的β-δ 细胞对。
Physically interacting beta-delta pairs in the regenerating pancreas revealed by single-cell sequencing.
机构信息
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel; Department of Molecular Neuroscience, Weizmann Institute of Science, Rehovot 7610001, Israel.
Department of Immunology, Weizmann Institute of Science, Rehovot 7610001, Israel.
出版信息
Mol Metab. 2022 Jun;60:101467. doi: 10.1016/j.molmet.2022.101467. Epub 2022 Mar 1.
OBJECTIVES
Until recently, communication between neighboring cells in islets of Langerhans was overlooked by genomic technologies, which require rigorous tissue dissociation into single cells.
METHODS
We utilize sorting of physically interacting cells (PICs) with single-cell RNA-sequencing to systematically map cellular interactions in the endocrine pancreas after pancreatectomy.
RESULTS
The pancreas cellular landscape features pancreatectomy associated heterogeneity of beta-cells, including an interaction-specific program between paired beta and delta-cells.
CONCLUSIONS
Our analysis suggests that the particular cluster of beta-cells that pairs with delta-cells benefits from stress protection, implying that the interaction between beta- and delta-cells might safeguard against pancreatectomy associated challenges. The work encourages testing the potential relevance of physically-interacting beta-delta-cells also in diabetes mellitus.
目的
直到最近，基因组技术还忽略了胰岛中相邻细胞之间的通讯，因为这些技术需要严格地将组织解离成单个细胞。
方法
我们利用物理相互作用细胞（PICs）的分选，结合单细胞 RNA 测序，系统地绘制了胰腺切除术后内分泌胰腺中的细胞相互作用图谱。
结果
胰腺细胞景观的特征是β细胞与胰腺切除术相关的异质性，包括配对β和δ细胞之间的特异性相互作用程序。
结论
我们的分析表明，与δ细胞配对的β细胞的特定簇受益于应激保护，这意味着β和δ细胞之间的相互作用可能有助于抵御与胰腺切除术相关的挑战。这项工作鼓励在糖尿病中测试物理相互作用的β-δ细胞的潜在相关性。
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