使用 Ga-NOTA-c(NGR) 对手术大鼠模型中缺血/再灌注介导的氨肽酶 N 表达进行成像。

Imaging of Ischemia/Reperfusion-mediated Aminopeptidase N Expression in Surgical Rat Model Using Ga-NOTA-c(NGR).

机构信息

Division of Nuclear Medicine and Translational Imaging, Department of Medical Imaging, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.

Gyula Petrányi Doctoral School of Allergy and Clinical Immunology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.

出版信息

In Vivo. 2022 Mar-Apr;36(2):657-666. doi: 10.21873/invivo.12750.

DOI:10.21873/invivo.12750

PMID:35241519

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8931911/

Abstract

BACKGROUND/AIM: Previous studies have already shown that Gallium(Ga)-labeled NGR-based radiopharmaceuticals specifically bind to the neoangiogenic molecule Aminopeptidase N (APN/CD13). The aim of this study was to evaluate the applicability of Ga-NOTA-c(NGR) in the in vivo detection of the temporal changes of APN/CD13 expression in the diabetic retinopathy rat model using positron emission tomography (PET).

MATERIALS AND METHODS

Ischemia/reperfusion injury was initiated by surgical ligation of the left bulbus oculi of rats. In vivo PET imaging studies were performed after the surgery using Ga-NOTA-c(NGR).

RESULTS

Significantly higher Ga-NOTA-c(NGR) uptake was observed in the surgically-ligated left bulbus, compared to the bulbus of the non-surgical group at each investigated time point. The western blot and histological analysis confirmed the increased expression of the neo-angiogenic marker APN/CD13.

CONCLUSION

Ga-NOTA-c(NGR) is a suitable radiotracer for the detection of the temporal changes of the ischemia/reperfusion-mediated expression of APN/CD13 in the surgically induced diabetic retinopathy rat model.

摘要

背景/目的：之前的研究已经表明，镓（Ga）标记的基于 NGR 的放射性药物特异性结合新生血管生成分子氨基肽酶 N（APN/CD13）。本研究旨在评估 Ga-NOTA-c（NGR）在使用正电子发射断层扫描（PET）检测糖尿病视网膜病变大鼠模型中 APN/CD13 表达的时间变化中的适用性。