新型局部骨转移小鼠乳腺癌模型的建立及双膦酸盐的验证
Development of New Mouse Breast Cancer Model of Local Bone Metastasis and Verification Using Bisphosphonates.
机构信息
Department of Orthopedic Surgery, Akita University Graduate School of Medicine, Akita, Japan
Department of Orthopedic Surgery, Akita University Graduate School of Medicine, Akita, Japan.
出版信息
In Vivo. 2022 Mar-Apr;36(2):667-671. doi: 10.21873/invivo.12751.
BACKGROUND/AIM: Local tumor injection models require complicated procedures. The purpose was to establish a simple local bone metastasis model using normal mice, and to study the usefulness of the model with bisphosphonates (BP).
MATERIALS AND METHODS
This study used a versatile C57BL/6 mouse model and E0771 cells. Tumor cells were injected into the right femur. Mice were divided into groups depending on the concentration of cells injected and the use of BP or not. The degree of bone destruction between the different conditions was compared using micro-computed tomography (μCT).
RESULTS
Bone destruction was confirmed in four mice in the high-concentration group at 3 weeks, and in all other mice at 4 and 6 weeks. At 6 weeks post-injection, bone destruction was significantly suppressed in the BP group (p<0.05).
CONCLUSION
We created a breast cancer mouse model of local bone metastasis. Zoledronate showed the same usefulness as in previous models. It may be an effective model for evaluating treatments for bone metastasis.
背景/目的：局部肿瘤注射模型需要复杂的程序。本研究旨在使用普通小鼠建立一种简单的局部骨转移模型，并研究双膦酸盐 (BP) 在该模型中的应用价值。
材料和方法
本研究使用了一种多功能 C57BL/6 小鼠模型和 E0771 细胞。将肿瘤细胞注射到右侧股骨中。根据注射细胞的浓度以及是否使用 BP 将小鼠分为不同组。使用微计算机断层扫描 (μCT) 比较不同条件下的骨破坏程度。
结果
在高浓度组的 4 只小鼠中，在 3 周时确认有骨破坏，而在其他所有小鼠中，在 4 周和 6 周时均观察到骨破坏。在注射后 6 周时，BP 组的骨破坏明显受到抑制（p<0.05）。
结论
我们成功建立了一种局部骨转移的乳腺癌小鼠模型。唑来膦酸显示出与以往模型相同的有效性。该模型可能是评估骨转移治疗方法的有效模型。
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