开发一种新的肾细胞癌骨转移局灶性小鼠模型。
Development of a New Focal Mouse Model of Bone Metastasis in Renal Cell Carcinoma.
机构信息
Department of Orthopedic Surgery, Akita University Graduate School of Medicine, Akita, Japan;
Department of Orthopedic Surgery, Akita University Graduate School of Medicine, Akita, Japan.
出版信息
In Vivo. 2024 May-Jun;38(3):1074-1078. doi: 10.21873/invivo.13541.
BACKGROUND/AIM: Developing animal models of bone metastasis in renal cell carcinoma (RCC) is challenging as immunodeficient mice are required. The aim of this study was to develop a simple immune model of RCC bone metastasis.
MATERIALS AND METHODS
RENCA tumor cells were injected into the right femurs of BALB/c mice. Sixty mice were grouped into each twenty-mouse group according to the tumor cell concentration, and the presence or absence and extent of bone metastasis in the total length of the femur were compared using hematoxylin and eosin staining of the excised tissues.
RESULTS
Bone metastasis was significantly higher in the high concentration group than in the other groups (p<0.05), with 10 mice developing bone metastasis at two weeks and nine mice developing bone metastasis at three weeks. The extent of bone metastasis was significantly greater in the high concentration group than in the other groups (p<0.05). Multiple logistic regression analysis was performed to examine the factors influencing bone metastasis, and only the high concentration was a significant factor (p<0.05).
CONCLUSION
We developed a normal immunity mouse model of local bone metastasis from RCC. This model could prove valuable for research into the treatment of bone metastases in RCC.
背景/目的：由于需要免疫缺陷小鼠，因此开发肾细胞癌（RCC）骨转移的动物模型具有挑战性。本研究旨在建立 RCC 骨转移的简单免疫模型。
材料和方法
将 RENCA 肿瘤细胞注入 BALB/c 小鼠的右侧股骨中。根据肿瘤细胞浓度，将 60 只小鼠分为每组 20 只，通过对切除组织进行苏木精和伊红染色，比较各组总股骨中骨转移的存在与否及其程度。
结果
高浓度组的骨转移明显高于其他组（p<0.05），其中 10 只小鼠在 2 周时发生骨转移，9 只小鼠在 3 周时发生骨转移。高浓度组的骨转移程度明显大于其他组（p<0.05）。进行多因素逻辑回归分析以检查影响骨转移的因素，结果发现只有高浓度是显著因素（p<0.05）。
结论
我们建立了一种具有正常免疫功能的 RCC 局部骨转移小鼠模型。该模型可能对研究 RCC 骨转移的治疗具有重要价值。
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