Vanini Jaqueline Vaz, Koyama Leonardo Kenji Sakaue, de Matos Leandro Luongo, Junior José Martins Figueredo, Cernea Claudio Roberto, Nagano Cibele Pidorodeski, Coutinho-Camillo Cláudia Malheiros, Hsieh Ricardo, Lourenço Silvia Vanessa
Department of Stomatology, Dental School, University of São Paulo, São Paulo, Brazil.
Head and Neck Surgery Discipline, Medical School, University of São Paulo, São Paulo, Brazil.
J Bone Oncol. 2022 Feb 18;33:100418. doi: 10.1016/j.jbo.2022.100418. eCollection 2022 Apr.
Bone invasion is an important prognostic factor in oral squamous cell carcinoma, leading to a lower survival rate and the use of aggressive treatment approaches. Epithelial-mesenchymal transition (EMT) is possibly involved in this process, because it is often related to mechanisms of cell motility and invasiveness. This study examined whether a panel of epithelial-mesenchymal markers are present in cases of oral squamous cell carcinoma with bone invasion and whether these proteins have any relationship with patients' clinical-pathological parameters and prognostic factors.
Immunohistochemical analysis of E-cadherin, , vimentin, TGFβ1, and periostin was performed in paraffin-embedded samples of 62 oral squamous cell carcinoma cases.
The analysis revealed that most cases (66%) presented with a dominant tumor infiltrative pattern in bone tissue, associated with lower survival rates, when compared with cases with a dominant erosive invasion pattern (P = 0.048). Twenty-seven cases (43%) expressed markers that were compatible with total or partial EMT at the tumor-bone interface. There was no association between evidence of total or partial EMT and other demographic or prognostic features. E-cadherin-positive cases were associated with tobacco smoking (P = 0.022); vimentin-positive cases correlated with tumors under 4 cm (P = 0.043). expression was observed in tumors with a dominant infiltrative pattern (P = 0.041) and was associated with the absence of periostin (P = 0.031).
We observed evidence of total or partial EMT in oral squamous cell carcinoma bone invasion. The transcription factor appears to be involved in bone invasion and disease progression.
骨侵袭是口腔鳞状细胞癌的一个重要预后因素，会导致生存率降低并需要采用积极的治疗方法。上皮-间质转化（EMT）可能参与了这一过程，因为它通常与细胞运动和侵袭机制相关。本研究检测了一组上皮-间质标志物在伴有骨侵袭的口腔鳞状细胞癌病例中是否存在，以及这些蛋白与患者的临床病理参数和预后因素是否存在任何关系。
对62例口腔鳞状细胞癌病例的石蜡包埋样本进行E-钙黏蛋白、波形蛋白、转化生长因子β1（TGFβ1）和骨膜蛋白的免疫组织化学分析。
分析显示，与主要为侵蚀性侵袭模式的病例相比，大多数病例（66%）在骨组织中呈现主要的肿瘤浸润模式，生存率较低（P = 0.048）。27例（43%）病例在肿瘤-骨界面表达与完全或部分EMT相符的标志物。完全或部分EMT的证据与其他人口统计学或预后特征之间无关联。E-钙黏蛋白阳性病例与吸烟有关（P = 0.022）；波形蛋白阳性病例与直径小于4 cm的肿瘤相关（P = 0.043）。在主要为浸润性模式的肿瘤中观察到[具体蛋白名称未给出]表达（P = 0.041），且与骨膜蛋白缺失有关（P = 0.031）。
我们在口腔鳞状细胞癌骨侵袭中观察到完全或部分EMT的证据。转录因子[具体转录因子名称未给出]似乎参与了骨侵袭和疾病进展。
