维生素D缺乏对大鼠RANKL/OPG比值的影响。
The effect of vitamin D deficiency on the RANKL/OPG ratio in rats.
作者信息
Khalaf Rawan M, Almudhi Abdullazez A
机构信息
Department of Pediatric Dentistry and Orthodontics, College of Dentistry, King Saud University, Graduate Studies and Scientific Research, P.O.Box 60169-15, Riyadh, 11545, Saudi Arabia.
Division of Orthodontics, Department of Pediatric Dentistry and Orthodontics, College of Dentistry, Graduate Studies and Scientific Research, P.O.Box 60169-15, Riyadh, 11545, Saudi Arabia.
出版信息
J Oral Biol Craniofac Res. 2022 Mar-Apr;12(2):228-232. doi: 10.1016/j.jobcr.2022.02.004. Epub 2022 Feb 22.
The aim of this study was to determine the effect of vitamin D deficiency on the RANKL/OPG ((Receptor Activator of Nuclear Factor Kappa B Ligand/Osteoprotegerin) ratio in the serum blood levels. Sixteen Wistar rats were divided into 2 groups: a control group (C) and an experimental group (E). The group C comprised of rats with average vitamin D levels, while vitamin D deficiency was induced in those of group E. A test period of 21 days was employed wherein two serum blood samples were obtained, at the baseline (day 0) and at day 21. Changes in RANKL andOPG levels were measured using Enzyme-linked immunosorbent assay (ELISA) kits. A statistically significant decrease of serum RANKL concentration and RANKL/OPG ratio on day 21 was seen in the experimental group compared to the control group. The serum RANKL levels and RANKL/OPG ratio in rats, were negatively affected by the deficiency of vitamin D.
本研究的目的是确定维生素D缺乏对血清中核因子κB受体活化因子配体/骨保护素（RANKL/OPG）比值的影响。将16只Wistar大鼠分为2组：对照组（C）和实验组（E）。C组由维生素D水平正常的大鼠组成，而E组大鼠则诱导维生素D缺乏。采用21天的试验期，在基线（第0天）和第21天采集两份血清样本。使用酶联免疫吸附测定（ELISA）试剂盒测量RANKL和OPG水平的变化。与对照组相比，实验组在第21天时血清RANKL浓度和RANKL/OPG比值有统计学意义的下降。大鼠血清中的RANKL水平和RANKL/OPG比值受到维生素D缺乏的负面影响。
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