Steele R E, Preston A S, Johnson J P, Handler J S
Am J Physiol. 1986 Jul;251(1 Pt 1):C136-9. doi: 10.1152/ajpcell.1986.251.1.C136.
Porous-bottom dishes offer several advantages for growing and studying epithelia in culture. Many epithelia differentiate more on porous surfaces than on plastic tissue culture dishes. In addition, separate solutions can be maintained on each side of the epithelium and can be sampled easily for studies of transport and other polarized functions. We describe the fabrication of dishes with a cellulose ester filter, a collagen-coated polycarbonate filter, or a collagen membrane forming the surface for cell attachment at the bottom of the dish.
多孔底部培养皿在培养和研究上皮细胞方面具有几个优点。许多上皮细胞在多孔表面比在塑料组织培养皿上分化得更好。此外，上皮细胞两侧可以维持不同的溶液，并且可以很容易地取样用于研究运输和其他极化功能。我们描述了用纤维素酯滤膜、胶原包被的聚碳酸酯滤膜或胶原膜制作培养皿的方法，这些材料形成了培养皿底部用于细胞附着的表面。
