Whitlon D S, Baas P W
Waisman Center, University of Wisconsin, Madison 53705.
J Histochem Cytochem. 1992 Jun;40(6):875-7. doi: 10.1177/40.6.1588032.
For many applications, cells or tissue must be cultured on an optical surface of high quality. For such applications laboratories often prepare "special dishes," which are made by affixing a glass coverslip beneath a hole in a plastic petri dish bottom. In this report, we offer an improved method, using Parafilm as a dry mount adhesive, for the preparation of special dishes, and show that the resulting dish is non-toxic to neurons in culture. The Parafilm bond is stable at 60 degrees C, permitting electron microscopy resins to be poured directly into the dishes and cured. The glass coverslip can be readily removed from the cured resin mechanically. The techniques we describe offer time-saving and reliable improvements for the use of glass coverslips in cell culture and electron microscopy.
对于许多应用而言，细胞或组织必须在高质量的光学表面上进行培养。针对此类应用，实验室通常会制备“特殊培养皿”，其制作方法是在塑料培养皿底部的一个孔下方粘贴一块玻璃盖玻片。在本报告中，我们提供了一种改进方法，使用石蜡膜作为干式固定粘合剂来制备特殊培养皿，并表明所得到的培养皿对培养中的神经元无毒。石蜡膜粘结在60摄氏度时稳定，可使电子显微镜树脂直接倒入培养皿中并固化。固化后的树脂中，玻璃盖玻片可以很容易地通过机械方式移除。我们所描述的技术为细胞培养和电子显微镜中玻璃盖玻片的使用提供了省时且可靠的改进方法。
