Paller M S, Hostetter T H
Am J Physiol. 1986 Jul;251(1 Pt 2):F34-9. doi: 10.1152/ajprenal.1986.251.1.F34.
The effect of dietary protein on the renin-angiotensin system was studied in rats. Rats were fed isocaloric, 50% (high protein, HP), or 6% (low protein, LP) protein diets with identical electrolyte content for 10 days. Food intake and electrolyte excretion were equivalent on the two diets. Plasma renin activity (PRA) was higher in HP (10.0 +/- 2.5 vs. 3.5 +/- 0.5 ng ANG I . ml-1 . h-1, P less than 0.02) as was plasma aldosterone. However, in conscious rats mean arterial pressure (MAP) was not different between groups. The pressor response to graded doses of angiotensin II (ANG II) was diminished by 30-60% with HP (all doses, P less than 0.05). ANG II binding by mesenteric artery smooth muscle particles did not differ between HP and LP. Chronic administration of captopril did not normalize the pressor response in HP. Urinary prostaglandin (PG) E and 6-keto-PGF1 alpha excretion was markedly increased by the HP diet. Acute inhibition of prostaglandin synthesis with meclofenamate restored the pressor response to ANG II in HP to that in LP. In summary, a HP diet increased PRA, plasma aldosterone, urinary PGE, and 6-keto-PGF1 alpha and decreased pressor responsiveness to ANG II. Resistance to ANG II was not reversed by chronic converting enzyme inhibition but was abolished by inhibition of prostaglandin synthesis.
在大鼠中研究了膳食蛋白质对肾素 - 血管紧张素系统的影响。给大鼠喂食等热量、蛋白质含量为50%（高蛋白，HP）或6%（低蛋白，LP）且电解质含量相同的饮食，持续10天。两种饮食的食物摄入量和电解质排泄量相当。高蛋白组的血浆肾素活性（PRA）较高（10.0±2.5对3.5±0.5 ng血管紧张素I·ml⁻¹·h⁻¹，P<0.02），血浆醛固酮水平也是如此。然而，清醒大鼠的平均动脉压（MAP）在各组之间并无差异。高蛋白饮食使对不同剂量血管紧张素II（ANG II）的升压反应降低了30 - 60%（所有剂量，P<0.05）。高蛋白组和低蛋白组肠系膜动脉平滑肌颗粒对ANG II的结合没有差异。长期给予卡托普利并不能使高蛋白组的升压反应恢复正常。高蛋白饮食显著增加了尿中前列腺素（PG）E和6 - 酮 - PGF1α的排泄。用甲氯芬那酸急性抑制前列腺素合成可使高蛋白组对ANG II的升压反应恢复到低蛋白组的水平。总之，高蛋白饮食增加了PRA、血浆醛固酮、尿中PGE和6 - 酮 - PGF1α，并降低了对ANG II的升压反应性。慢性抑制转化酶并不能逆转对ANG II的抵抗，但抑制前列腺素合成可消除这种抵抗。
