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以主食谷物和蜂幼虫()混合而成的补充食物喂养的BALB/c小鼠的生长性能、生化和血液学参数。

Growth performance, biochemical and haematological parameters of BALB/c mice fed on staple grains and bee larvae () blended complementary foods.

作者信息

Mekuria Shewangzaw Addisu, Kinyuru John N, Mokua Beatrice Kiage, Tenagashaw Mesfin Wogayehu

机构信息

Department of Food Science and Nutrition, Jomo Kenyatta University of Agriculture and Technology, P.O. Box 62000-00200, Nairobi, Kenya.

University of Gondar, P.O. Box 196, Gondar, Ethiopia.

出版信息

Heliyon. 2022 Feb 24;8(2):e09003. doi: 10.1016/j.heliyon.2022.e09003. eCollection 2022 Feb.

Abstract

In Sub-Saharan Africa, inadequate complementary feeding practices and being nutritionally inadequate are primary factors in infant and young child malnutrition, growth failure, and high morbidity and mortality. Therefore, novel complementary foods need to be developed to alleviate malnutrition problems in IYC. Therefore, this experimental study aimed to assess the effects of newly developed grain-bee larvae blended complementary foods on the growth performance, haematological, and biochemical parameters of BALB/c mice. A complete randomized design was used and a total of 75 BALB/c mice were assigned to each of the five treatments. The treatments were: T1 = Casein diet; T2 = 57 % Maize, 29 % Teff, 14 % Soybean; T3 = 58 % Maize, 29 % Teff, 13 % Bee larvae; T4 = Commercial wean mix; and T5 = Basal diet alone. The experiment trial was done for 28 days along with seven days of adaptation. Dietary intake was not significantly different (P = 0.96) between treatments, but it was noted that T3 had gained the highest final body weight (38.52 g). The examined biochemical parameters showed T4 had the lowest serum protein (6.27 mg/dl) and globulin (3.61 mg/dl). Compared to others, T3 significantly (P < 0.001) increased WBC (4 × 10 mm), RBC (11.37 × 10 mm), Haemoglobin (16.42 g/dl), and Hematocrit (63.04 %). The highest serum levels of zinc (0.55 mg/dl) and iron (2.08 mg/dl) were reported on T2, while the highest serum calcium content (10.64 mg/dl) was reported on T1. The results indicated that T3 can aid body growth, health, and prevent malnutrition in infants and young children.

摘要

在撒哈拉以南非洲地区,不适当的辅食喂养方式以及营养不足是婴幼儿营养不良、生长发育迟缓以及高发病率和高死亡率的主要因素。因此,需要开发新型辅食来缓解婴幼儿的营养不良问题。因此,本实验研究旨在评估新开发的谷物-蜂幼虫混合辅食对BALB/c小鼠生长性能、血液学和生化参数的影响。采用完全随机设计,将75只BALB/c小鼠分配到五种处理组中。处理组分别为:T1 = 酪蛋白饮食;T2 = 57%玉米、29%画眉草、14%大豆;T3 = 58%玉米、29%画眉草、13%蜂幼虫;T4 = 商业断奶混合物;T5 = 仅基础饮食。实验为期28天,外加7天适应期。各处理组之间的饮食摄入量无显著差异(P = 0.96),但注意到T3组的最终体重最高(38.52克)。检测的生化参数显示,T4组的血清蛋白(6.27毫克/分升)和球蛋白(3.61毫克/分升)最低。与其他组相比,T3组显著(P < 0.001)增加了白细胞(4×10⁹/毫米)、红细胞(11.37×10¹²/毫米)、血红蛋白(16.42克/分升)和血细胞比容(63.04%)。T2组报告的血清锌水平最高(0.55毫克/分升)和铁水平最高(2.08毫克/分升),而T1组报告的血清钙含量最高(10.64毫克/分升)。结果表明,T3有助于婴幼儿的身体生长、健康并预防营养不良。

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