高通量细胞术染色人类骨髓临床样本。

Mass cytometry staining for human bone marrow clinical samples.

机构信息

Department of Medical Oncology, Center for Immuno-Oncology, Dana-Farber Cancer Institute, 450 Brookline Avenue, Boston, MA 02215, USA.

Center for Prevention of Progression of Blood Cancers, Dana-Farber Cancer Institute, 450 Brookline Avenue, Boston, MA 02215, USA.

出版信息

STAR Protoc. 2022 Feb 17;3(1):101163. doi: 10.1016/j.xpro.2022.101163. eCollection 2022 Mar 18.

DOI:10.1016/j.xpro.2022.101163

PMID:35243367

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8861824/

Abstract

This protocol details a staining technique optimized for immunophenotyping of human bone marrow immune populations using mass cytometry. The protocol accounts for the limitations of working with human bone marrow, such as reduced viability, low cell counts, and fragile cell pellets, to successfully acquire single viable cells ready for downstream analysis. This assay can be used to characterize the activation, exhaustion, and cytotoxicity of immune populations and ensure comprehensive immunophenotyping of human bone marrow clinical samples.

摘要

本方案详细介绍了一种使用液质联用技术对人骨髓免疫细胞群体进行免疫表型分析的染色技术。该方案考虑到了与人骨髓样本相关的一些限制因素，如细胞活力降低、细胞计数低以及脆弱的细胞沉淀等，以成功获取适合下游分析的单个活细胞。该检测方法可用于分析免疫细胞群体的激活、耗竭和细胞毒性，并确保全面分析人骨髓临床样本的免疫表型。