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一种通过流式细胞术检测小鼠抗体分泌浆细胞亚群的新染色方案。

A new staining protocol for detection of murine antibody-secreting plasma cell subsets by flow cytometry.

作者信息

Pracht Katharina, Meinzinger Julia, Daum Patrick, Schulz Sebastian R, Reimer Dorothea, Hauke Manuela, Roth Edith, Mielenz Dirk, Berek Claudia, Côrte-Real Joana, Jäck Hans-Martin, Schuh Wolfgang

机构信息

Division of Molecular Immunology, Internal Medicine III, Nikolaus-Fiebiger-Center of Molecular Medicine, University Hospital Erlangen, Erlangen, Germany.

German Rheumatism Research Center (DRFZ) Berlin, Berlin, Germany.

出版信息

Eur J Immunol. 2017 Aug;47(8):1389-1392. doi: 10.1002/eji.201747019. Epub 2017 Jul 13.

DOI:10.1002/eji.201747019
PMID:28608550
Abstract

We provide a robust four-color fluorescence-based flow cytometry protocol that distinguishes viable dividing plasmablasts from nondividing plasma cells and, based on CD19 surface abundance, identifies two mature plasma cell populations in the spleen and the bone marrow of mice.

摘要

我们提供了一种基于四色荧光的强大流式细胞术方案，该方案可区分存活的增殖浆母细胞与非增殖浆细胞，并根据CD19表面丰度，识别小鼠脾脏和骨髓中的两个成熟浆细胞群体。

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