Department of Neurobiology, Institute of Life Sciences, Edmond J. Safra Campus, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Okinawa Institute of Science and Technology, Graduate University, Okinawa 904 0495, Japan.
STAR Protoc. 2022 Feb 21;3(1):101192. doi: 10.1016/j.xpro.2022.101192. eCollection 2022 Mar 18.
Due to their unique body, standard behavioral testing protocols are often hard to apply to octopuses. Our protocol enables controlled behavioral testing of the sensory systems in single arms while allowing observation of the arm motion. The protocol allows the researcher to exclude the sense of vision without surgical manipulation and selectively test peripheral sensory input-derived learning and motor behavior. Applying the protocol requires systematic and multistage training of octopuses to associate correct maze interaction with food reward. For complete details on the use and execution of this profile, please refer to Gutnick et al. (2020).
由于它们独特的身体结构，通常很难将标准的行为测试方案应用于章鱼。我们的方案使我们能够在控制单臂感觉系统的同时，观察手臂运动，从而进行受控的行为测试。该方案允许研究人员在不进行手术操作的情况下排除视觉感知，并选择性地测试外周感觉输入衍生的学习和运动行为。应用该方案需要对章鱼进行系统的、多阶段的训练，以使其将正确的迷宫互动与食物奖励联系起来。有关此方案的使用和执行的详细信息，请参考 Gutnick 等人（2020 年）。
