Guindo Cheick Oumar, Morsli Madjid, Bellali Sara, Drancourt Michel, Grine Ghiles
IHU Méditerranée Infection, Marseille, France.
Aix-Marseille-Université, IRD, MEPHI, IHU Méditerranée Infection, France.
Curr Res Microb Sci. 2022 Feb 16;3:100112. doi: 10.1016/j.crmicr.2022.100112. eCollection 2022.
() is a facultative anaerobic, coccus-shaped halophilic lactic acid-producing bacterium previously detected and cultured in various salty foods and credited for beneficial effects on human health. In this study, we investigated the presence of in human samples using a polyphasic approach including scanning electron microscopy, molecular biology methods and microbial culture. This unique investigation yielded the unprecedented presence of in human feces samples, thus enriching the repertoire of halophilic microorganisms colonizing the human gastrointestinal tract with the isolation and culture of for the first time in humans. Using the E-test strips, the MIC was assessed for T. strain CSURQ6002: rifampicin (MIC at 0.002 μg/mL), benzylpenicillin (MIC at 0.094 μg/mL), amoxicillin (MIC at 0.5 μg/mL), erythromycin (MIC at 2 μg/mL), clindamycin (MIC at 4 μg/mL), and vancomycin (MIC at 8 μg/mL). However, this strain showed a MIC up to 256 μg/mL for ciprofloxacin, fosfomycin, doxycyclin, imipenem, and colistin. profiling derived from whole genome sequencing (NCBI accession number: PRJNA780809), was confirmed. This discovery suggested that was part of the human digestive microbiota and that its potential role on human health should be considered.
（）是一种兼性厌氧、球菌状的嗜盐产乳酸菌，此前在各种咸味食品中被检测和培养，并被认为对人类健康有益。在本研究中，我们采用多相方法，包括扫描电子显微镜、分子生物学方法和微生物培养，来调查人类样本中（）的存在情况。这项独特的研究首次在人类粪便样本中发现了（），这是前所未有的，通过在人类中首次分离和培养（），丰富了定殖于人类胃肠道的嗜盐微生物种类。使用E-test试纸条，对（）菌株CSURQ6002的最低抑菌浓度（MIC）进行了评估：利福平（MIC为0.002μg/mL）、苄青霉素（MIC为0.094μg/mL）、阿莫西林（MIC为0.5μg/mL）、红霉素（MIC为2μg/mL）、克林霉素（MIC为4μg/mL）和万古霉素（MIC为8μg/mL）。然而，该菌株对环丙沙星、磷霉素、强力霉素、亚胺培南和黏菌素的MIC高达256μg/mL。源自全基因组测序（NCBI登录号：PRJNA780809）的（）基因图谱得到了证实。这一发现表明（）是人类消化微生物群的一部分，其对人类健康的潜在作用应予以考虑。
