从韩国大豆酱曲中分离得到的嗜盐四联球菌 EFEL7002 的益生菌潜力。

Probiotic potential of Tetragenococcus halophilus EFEL7002 isolated from Korean soy Meju.

机构信息

Brain Korea 21 Center for Bio-Health Industry, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University, Cheongju, 28644, Republic of Korea.

Department of Life Science, Chung-Ang University, Seoul, 156-756, Republic of Korea.

出版信息

BMC Microbiol. 2022 Jun 6;22(1):149. doi: 10.1186/s12866-022-02561-7.

BACKGROUND

Probiotic starters can improve the flavor profile, texture, and health-promoting properties of fermented foods. Tetragenococcus halophilus is a halophilic lactic acid bacterium that is a candidate starter for high-salt fermented foods. However, the species is known to produce biogenic amines, which are associated with neurotoxicity. Here, we report a probiotic starter strain of T. halophilus, EFEL7002, that is suitable for use in high-salt fermentation.

RESULTS

EFEL7002 was isolated from Korean meju (fermented soybean) and identified as T. halophilus, with 99.85% similarity. The strain is safe for use in food as it is a non-hemolytic and non-biogenic amine producer. EFEL7002 is tolerant to gastrointestinal conditions and can adhere to Caco-2 cells. This strain showed antioxidant, anti-inflammatory, and protective effects against the human gut epithelial barrier. EFEL7002 grew well in media containing 0-18% NaCl showing maximum cell densities in 6% or 12% NaCl.

CONCLUSIONS

T. halophilus EFEL7002 can be used as a health-promoting probiotic starter culture for various salty fermented foods.

背景

益生菌发酵剂可以改善发酵食品的风味、质地和促进健康的特性。四氢嗜热链球菌是一种嗜盐乳酸菌，是高盐发酵食品的候选发酵剂。然而，该物种已知会产生生物胺，与神经毒性有关。在这里，我们报告了一种适用于高盐发酵的益生菌发酵剂菌株 Tetragenococcus halophilus EFEL7002。

结果

EFEL7002 从韩国豆酱（发酵大豆）中分离出来，被鉴定为 Tetragenococcus halophilus，相似度为 99.85%。该菌株是一种非溶血和非生物胺产生菌，可安全用于食品。EFEL7002 耐受胃肠道条件，并能黏附于 Caco-2 细胞。该菌株具有抗氧化、抗炎和保护人类肠道上皮屏障的作用。EFEL7002 在含有 0-18%NaCl 的培养基中生长良好，在 6%或 12%NaCl 中达到最大细胞密度。