[用于利什曼原虫前鞭毛体和无鞭毛体形式生产的培养系统。在血清学诊断和治疗试验中的应用]

[Culture systems for production of promastigote and amastigote forms of Leishmania. Application to serological diagnosis and therapeutic trials].

作者信息

Vouldoukis I, Alfred C, Monjour L, Mazier D, Brandicourt O, Ploton I, Tselentis Y, Nzuzi K K, Gentilini M

出版信息

Ann Parasitol Hum Comp. 1986;61(2):147-54. doi: 10.1051/parasite/1986612147.

DOI:10.1051/parasite/1986612147

Abstract

Several species of leishmania and three methods of cultivation: monophasic, biphasic and co-cultivation were used in a compared study bearing on the intensive production of leishmania. In addition by applying, a new in vivo model, comprising an injection of sarcomatous cells and promastigotes into BALB/c mice and also an extraction on a discontinuous gradient (Radioselectan 60%), it was possible to obtain highly purified isolates of amastigote forms. The use of two antigens: promastigotes and amastigotes, is to be recommended for the serological diagnosis, by indirect immunofluorescence, of kala-azar. The new in vivo model merits further consideration for research concerning new molecules active against leishmania.

摘要

在一项关于利什曼原虫集约化生产的对比研究中，使用了几种利什曼原虫物种以及三种培养方法：单相培养、双相培养和共培养。此外，通过应用一种新的体内模型，即将肉瘤细胞和前鞭毛体注射到BALB/c小鼠体内，并在不连续梯度（Radioselectan 60%）上进行提取，有可能获得高度纯化的无鞭毛体形式的分离物。推荐使用两种抗原：前鞭毛体和无鞭毛体，通过间接免疫荧光法进行黑热病的血清学诊断。对于研究抗利什曼原虫的新活性分子，这种新的体内模型值得进一步考虑。