Deletre Emilie, Matu Francis Kiamba, Murungi Lucy Kananu, Mohamed Samira
CIRAD-UPR HORTSYS, University of Montpellier, TA B-103/C-Campus International de Baillarguet, 34398 Montpellier Cedex, France.
International Center of Insect Physiology and Ecology, Nairobi, Kenya.
J Econ Entomol. 2022 Apr 13;115(2):565-572. doi: 10.1093/jee/toac015.
The greenhouse whitefly, Trialeurode vaporariorum, is among the key pests of tomato (Solanum lycopersicum) in sub-Saharan Africa with Tuta absoluta, spider mite, thrips, and fruitworms. To understand the interaction between the pest and the plant's herbivory-induced plant volatile (HIPVs), we investigated the repellency of four tomato cultivars (Kilele F1, Assila F1, Red Beauty F1, and Nemonneta F1) upon infestation by Trialeurode vaporariorum. We analyzed the behavioral response of T. vaporariorum to infested and uninfested tomato plants of these cultivars using olfactory bioassays followed by gas chromatography-mass spectrometry (GC-MS) analyses of emitted volatiles. Trialeurode vaporariorum was attracted to uninfested plants of all four tomato cultivars. However, two cultivars Kilele F1 and Red Beauty F1 were no longer attractive to the whitefly when they were already infested by the pest. GC-MS analyses identified 25 compounds, 18 monoterpenes, 3 sesquiterpenes, 2 xylenes, 1 aldehyde, and 1 carboxylic compound in the 4 uninfested and infested cultivars. Based on the insects' behavioral response, 1,8-cineole, p-cymene, and limonene did not attract T. vaporariorum at varying concentrations when combined with Red Beauty F1, the most attractive tomato cultivar. This repellence behavioral response can be used as a basis for improvement of other vegetable crops for the management of arthropod pests as for odor masking technique.
温室白粉虱（Trialeurode vaporariorum）是撒哈拉以南非洲番茄（Solanum lycopersicum）的主要害虫之一，与番茄潜叶蛾、叶螨、蓟马和果虫一同构成危害。为了解这种害虫与植物食草诱导植物挥发物（HIPVs）之间的相互作用，我们研究了四种番茄品种（Kilele F1、Assila F1、Red Beauty F1和Nemonneta F1）在受到温室白粉虱侵害时的驱避性。我们使用嗅觉生物测定法分析了温室白粉虱对这些品种受侵害和未受侵害番茄植株的行为反应，随后对释放的挥发物进行了气相色谱 - 质谱（GC - MS）分析。温室白粉虱被所有四个番茄品种的未受侵害植株所吸引。然而，当Kilele F1和Red Beauty F1这两个品种已经受到害虫侵害时，对粉虱不再具有吸引力。GC - MS分析在4个未受侵害和受侵害的品种中鉴定出25种化合物，其中包括18种单萜、3种倍半萜、2种二甲苯、1种醛和1种羧酸化合物。基于昆虫的行为反应，当1,8 - 桉叶素、对伞花烃和柠檬烯与最具吸引力的番茄品种Red Beauty F1组合时，在不同浓度下均未吸引温室白粉虱。这种驱避行为反应可作为改进其他蔬菜作物以管理节肢动物害虫的基础手段，如气味掩盖技术。
