Darshanee Hewa L C, Ren Hui, Ahmed Nazeer, Zhang Zhan-Feng, Liu Yan-Hong, Liu Tong-Xian
State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F UniversityYangling, China.
Key Laboratory of Integrated Pest Management on the Loess Plateau of Ministry of Agriculture, Northwest A&F UniversityYangling, China.
Front Plant Sci. 2017 Jul 20;8:1285. doi: 10.3389/fpls.2017.01285. eCollection 2017.
The behavior of the greenhouse whitefly, Westwood (Hemiptera: Aleyrodidae), is known to be affected by plant volatile cues, but its attraction or repellent to specific volatile cues has not been deeply studied yet. Therefore, the aim of our study was to identify the most attractive plant among cultivars of tomato () and eggplant () to evaluate the volatiles of plants to identify the chemical compound(s) that attract . We speculated that whitefly-host plant interaction primarily depends on plant volatile emissions and that once the plant is damaged, it might attract more whiteflies. Three intact (uninfested) tomato, four intact eggplant cultivars and whitefly infested plants of the most whitefly attractive tomato and eggplant cultivars were examined by behavioral assay experiments for attractiveness to and headspace volatile were determined by solid-phase microextraction (SPME) and gas chromatography-mass spectrometry. Whiteflies had the highest preference for the intact eggplant Kuai Yuan Qie (KYQ) among the eggplant and the tomato plant cultivars in bioassay experiments. Although both male and female whiteflies were significantly more attracted to infested KYQ plants than to intact plants, whitefly females did not select conspecific-infested YG plants. The volatile emissions among different plant cultivars in individual species and infested versus intact plants were significantly different. Among these volatiles, identified major green leaf volatiles [(Z)-3-hexen-1-ol] and terpenoids [α-pinene, (E)-β-caryophyllene, α-humulene, azulene] showed a constitutive relationship with the most whitefly preference plants. Our findings provide new insights into the chemical compounds that attract or repel whiteflies.
烟粉虱，韦斯特伍德（半翅目：粉虱科）的行为已知会受到植物挥发性信号的影响，但其对特定挥发性信号的吸引或排斥作用尚未得到深入研究。因此，我们研究的目的是在番茄（）和茄子（）品种中找出最具吸引力的植物，以评估植物的挥发性物质，从而确定吸引烟粉虱的化学化合物。我们推测粉虱与寄主植物的相互作用主要取决于植物挥发性物质的排放，并且一旦植物受到损害，可能会吸引更多的粉虱。通过行为测定实验，对三个完整（未受侵染）的番茄品种、四个完整的茄子品种以及烟粉虱最具吸引力的番茄和茄子品种的受侵染植株进行了对烟粉虱吸引力的检测，并通过固相微萃取（SPME）和气相色谱 - 质谱联用技术测定了顶空挥发性物质。在生物测定实验中，烟粉虱对茄子品种中的完整茄子“快圆茄”（KYQ）和番茄品种表现出最高的偏好。虽然雄性和雌性烟粉虱对受侵染的KYQ植株的吸引力均显著高于完整植株，但粉虱雌性并未选择同种受侵染的YG植株。不同植物品种之间以及受侵染植株与完整植株之间的挥发性物质排放存在显著差异。在这些挥发性物质中，已鉴定出的主要绿叶挥发性物质[（Z）-3-己烯-1-醇]和萜类化合物[α-蒎烯、（E）-β-石竹烯、α-葎草烯、薁]与最受粉虱偏好的植物存在组成性关系。我们的研究结果为吸引或排斥粉虱的化学化合物提供了新的见解。
