Purification of cytoplasmic precursors of yeast mitochondrial phenylalanyl-tRNA synthetase subunits.
作者信息
Diatewa M, Taite J L, Stahl A J
出版信息
Biochem Biophys Res Commun. 1986 Jun 30;137(3):1119-24. doi: 10.1016/0006-291x(86)90341-4.
PMID:3524566
Abstract
Two polypeptidic precursors of yeast mitochondrial phenylalanyl-tRNA synthetase subunits were purified from the cytoplasm by immunoprecipitation with an insolubilized glutaraldehyde-treated IgG fraction, followed by two chromatographies on Sephadex G-200 and on DEAE-cellulose. Methionine was found as the N-terminal residue in both precursors, which exhibited N-terminal extensions.
摘要
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