Diatewa M, Stahl A J
Nucleic Acids Res. 1981 Dec 11;9(23):6293-304. doi: 10.1093/nar/9.23.6293.
The biosynthesis of yeast mitochondrial Phe-tRNA synthetase is studied in vivo. Antibodies against the enzyme are raised in rabbits. They precipitate two proteins in the post-ribosomal supernatant of the yeast cell homogenate. Immunoprecipitate analysis on SDS - gel electrophoresis shows that the two types of mitochondrial enzyme subunits with molecular weights of 57,000 and 72,000, respectively, are cytoplasmically synthesized as larger, individual precursors. Terminal extensions of the precursors prevent enzyme activity. Mitochondrial membranes linked protease(s) play(s) an active role in maturation.
对酵母线粒体苯丙氨酸 - 转运核糖核酸合成酶的生物合成进行了体内研究。用该酶对兔子进行免疫以产生抗体。这些抗体在酵母细胞匀浆的核糖体后上清液中沉淀出两种蛋白质。SDS - 凝胶电泳的免疫沉淀分析表明，两种分子量分别为57,000和72,000的线粒体酶亚基在细胞质中作为更大的单个前体合成。前体的末端延伸会抑制酶活性。与线粒体膜相连的蛋白酶在成熟过程中起积极作用。
