[凝血酶和胰蛋白酶对N-芳基磺酰基-缬氨酰-精氨酸甲酯水解作用的比较研究]
[Comparative study of hydrolysis of methyl esters of N-arylsulfonyl-valyl-arginine by thrombin and trypsin].
作者信息
Kibirev V K, Sereĭskaia A A, Romanova V P, Serebrianyĭ S B
出版信息
Biokhimiia. 1979 Apr;44(4):616-21.
PMID:35247
Abstract
The esterase action of thrombin and trypsin on N-arylsulfonyl-valyl-arginine methyl esters was studied. The values of Km and kcat under steady-state conditions at pH 8,5 were determined. It was shown that the nature of the arylsulfonyl group does not affect the kinetic parameters of the reactions under study. The Michaelis constants of the thrombin-catalyzed reactions appeared to be one order of magnitude lower than the Km values of the corresponding TAME analogs.
摘要
研究了凝血酶和胰蛋白酶对N-芳基磺酰基-缬氨酰-精氨酸甲酯的酯酶作用。测定了在pH 8.5的稳态条件下的Km和kcat值。结果表明，芳基磺酰基的性质不影响所研究反应的动力学参数。凝血酶催化反应的米氏常数似乎比相应的TAME类似物的Km值低一个数量级。
相似文献
1
2
3
[Hydrolysis of the methyl esters of the N-arylsulfonyl derivatives of L-arginine by thrombin and trypsin].[凝血酶和胰蛋白酶对L-精氨酸N-芳基磺酰基衍生物甲酯的水解作用]
Biokhimiia. 1975 Jan-Feb;40(1):103-6.
4
5
6
[Effect of temperature on the hydrolysis of some synthetic substrates by thrombin and trypsin].[温度对凝血酶和胰蛋白酶水解某些合成底物的影响]
Biokhimiia. 1981 Oct;46(10):1799-806.
7
[Kinetics of trypsin-catalyzed hydrolysis of some arginine-containing peptide methyl esters].[胰蛋白酶催化水解某些含精氨酸肽甲酯的动力学]
Ukr Biokhim Zh (1999). 2002 May-Jun;74(3):113-5.
8
9
[Comparative kinetic studies of the primary specificity of bovine and salmon trypsin].[牛和鲑鱼胰蛋白酶一级特异性的比较动力学研究]
Biokhimiia. 1992 Jan;57(1):55-60.
10
[Comparison of the catalytic properties of alpha- and beta-forms of thrombin].[凝血酶α型和β型催化特性的比较]
Biokhimiia. 1978;43(4):708-16.
Zotero 插件