Broudy V C, Zuckerman K S, Jetmalani S, Fitchen J H, Bagby G C
Blood. 1986 Aug;68(2):530-4.
In previous studies we have found that monocytes produce soluble factors that stimulate human umbilical vein endothelial cells to produce granulocyte-macrophage colony-stimulating activity (CSA), burst-promoting activity (BPA), and megakaryocyte colony-stimulating activity (Meg-CSA) as well as factors that stimulate T lymphocytes and neonatal fibroblasts to produce CSA. To test the hypothesis that monocytes would similarly stimulate the production of hematopoietic growth factors by autologous bone marrow stromal cells, multiply-passaged adherent fibroblastoid cells derived from the bone marrow of normal volunteers were exposed to conditioned media prepared by incubating autologous peripheral blood monocytes in complete medium for three days. When conditioned media from stromal cells incubated in monocyte-conditioned medium were compared with those of stromal cells cultured in the absence of monocyte-conditioned medium, BPA was increased fourfold and CSA was increased more than 30-fold. We conclude that mononuclear phagocytes recruit stromal cells of the marrow to produce multilineage growth factors in vitro. We suggest that these monocyte-derived recruiting activities may play an important role in orchestration of hematopoietic growth factor production by cells of the marrow microenvironment.
在先前的研究中,我们发现单核细胞可产生可溶性因子,这些因子能刺激人脐静脉内皮细胞产生粒细胞 - 巨噬细胞集落刺激活性(CSA)、爆式促进活性(BPA)和巨核细胞集落刺激活性(Meg - CSA),还能刺激T淋巴细胞和新生成纤维细胞产生CSA。为了验证单核细胞是否同样会刺激自体骨髓基质细胞产生造血生长因子这一假说,将来自正常志愿者骨髓的多次传代贴壁成纤维样细胞暴露于通过在完全培养基中孵育自体外周血单核细胞三天而制备的条件培养基中。当将在单核细胞条件培养基中孵育的基质细胞的条件培养基与未添加单核细胞条件培养基培养的基质细胞的条件培养基进行比较时,BPA增加了四倍,CSA增加了30多倍。我们得出结论,单核吞噬细胞可促使骨髓基质细胞在体外产生多谱系生长因子。我们认为,这些单核细胞衍生的募集活性可能在协调骨髓微环境细胞产生造血生长因子方面发挥重要作用。