构建猪急性肾损伤连续性血液透析滤过型循环模型。
Constructing a continuous hemodiafiltration-type circulatory model of acute kidney injury in pigs.
机构信息
Medical Technology and Material Laboratory, Research and Business Development Division, Asahi Kasei Medical Co., Ltd., Shizuoka, Japan.
Japan SLC, Inc., Shizuoka, Japan.
出版信息
Ther Apher Dial. 2022 Jun;26(3):507-514. doi: 10.1111/1744-9987.13826. Epub 2022 Mar 15.
INTRODUCTION
Animal-model experimental systems capable of reflecting the effects of devices for continuous renal replacement therapy (CRRT) on living organisms are limited; thus, aimed to construct an animal model of AKI-CRRT using pigs.
METHODS
Pigs were subjected to renal artery ischemia-reperfusion injury (IRI) and then to a maximum of 24 h of continuous hemodiafiltration (CHDF)-type CRRT.
RESULTS
Post-IRI, pigs' creatinine levels rose threefold, and they exhibited 24 h of anuria and clear aggravation of oxidative stress, demonstrating successful induction of AKI for CRRT. Post-CRRT, no significant changes in their vital signs or hematological parameters were observed. Creatinine and blood urea nitrogen clearance, as well as suppression of increases in oxidative stress, were also confirmed.
CONCLUSION
We believe that the use of our model can enable the preclinical evaluation of the effects of under-development CRRT devices on living organisms under conditions similar to those encountered in an actual clinical setting.
简介
能够反映连续肾脏替代治疗（CRRT）设备对生物体影响的动物模型实验系统有限；因此，目的是构建一种使用猪的 AKI-CRRT 动物模型。
方法
对猪进行肾动脉缺血再灌注损伤（IRI），然后进行最多 24 小时的连续血液透析滤过（CHDF）型 CRRT。
结果
IRI 后，猪的肌酐水平升高了三倍，出现了 24 小时的无尿，氧化应激明显加重，成功诱导了 CRRT 的急性肾损伤。CRRT 后，其生命体征或血液学参数无明显变化。还证实了肌酐和尿素氮清除率以及氧化应激增加的抑制作用。
结论
我们相信，我们的模型的使用可以使对开发中的 CRRT 设备对实际临床环境中遇到的类似条件下生物体的影响进行临床前评估。
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