Zangiyani Mahsa Siyavoshi, Livani Fatemeh, Faridnia Roghiyeh, Kalani Hamed
Clinical Research Development Center, Golestan University of Medical Sciences, Gorgan, Iran.
Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
J Parasit Dis. 2025 Mar;49(1):103-110. doi: 10.1007/s12639-024-01741-8. Epub 2024 Sep 23.
Currently, cutaneous leishmaniasis (CL) treatment is facing a problem due to the increase in drug resistance. Nanoparticles, due to their characteristics, are suitable candidates for disease treatment. The aim of this study is the in vitro evaluation of zinc nanoparticles coated with peppermint () essential oil on . The effects of different concentrations of Zn- and meglumine antimoniate (MA; as a positive control) were studied on the promastigote and amastigote of , and their selectivity index (SI) was evaluated. The peaks at 2361.02 cm (C-N stretching), 1213.78 cm (CO-NH stretching), and 1110.83 cm (C=O stretching) were attributed to Zn-. The inhibitory concentration 50 (IC) was 17.48 μg/mL for Zn- and 19.09 μg/mL for MA on promastigote ( = 0.47). It was 11.3 μg/mL for Zn- and 14.1 μg/mL for MA on amastigote ( = 0.79). Lower IC on amastigote for Zn- indicated better effectiveness on . compared to MA, but higher SI for MA (37.55) as compared to Zn- (18.9) ( = 0.0002), highlighting more safety for MA. Compared to MA, the anti-leishmanial effect of Zn- and its nontoxic effects on macrophages (SI > 10) show that Zn- can be a candidate for the treatment of CL and it should be further studied on animal models.
目前，由于耐药性增加，皮肤利什曼病（CL）的治疗面临一个问题。纳米颗粒因其特性，是疾病治疗的合适候选者。本研究的目的是对涂有薄荷（）精油的锌纳米颗粒进行体外评估。研究了不同浓度的Zn - 和葡甲胺锑酸盐（MA；作为阳性对照）对的前鞭毛体和无鞭毛体的影响，并评估了它们的选择性指数（SI）。在2361.02 cm（C - N伸缩）、1213.78 cm（CO - NH伸缩）和1110.83 cm（C = O伸缩）处的峰归因于Zn - 。对于前鞭毛体，Zn - 的半数抑制浓度（IC50）为17.48 μg/mL，MA为19.09 μg/mL（P = 0.47）。对于无鞭毛体，Zn - 为11.3 μg/mL，MA为14.1 μg/mL（P = 0.79）。Zn - 对无鞭毛体较低的IC50表明其对的有效性优于MA，但与Zn - （18.9）相比，MA的SI较高（37.55）（P = 0.0002），突出了MA更高的安全性。与MA相比，Zn - 的抗利什曼原虫作用及其对巨噬细胞的无毒作用（SI > 10）表明Zn - 可以作为CL治疗的候选者，应在动物模型上进一步研究。
