从牛颗粒性外阴炎中分离脲原体。
Isolation of Ureaplasma from bovine granular vulvitis.
作者信息
Ruhnke H L, Doig P A, MacKay A L, Gagnon A, Kierstead M
出版信息
Can J Comp Med. 1978 Apr;42(2):151-5.
Cultures for mycoplasmatales, viruses and bacteria were made from bovine vulvar swabs to determine whether ureaplasma was associated with a clinical granular vulvitis observed in 16 Ontario dairy herds. Ureaplasma was isolated from 23.5% of 34 clinically normal cows, 74% of 27 cows with mild to moderate vulvar hyperemia but no discharge and 100% of 20 cows with acute vulvar hyperemia accompanied by purulent discharge. There were statistically significant differences in rates of isolation among clinical groups. Mycoplasma bovigenitalium was isolated from 7.7% and 20% of cows with moderate or acute vulvitis respectively but not from normal cows. Haemophilus somnus was isolated from 25% of cows with acute vulvitis. There were no significant differences in isolations of Escherichia coli, Corynebacterium pyogenes and alpha-hemolytic streptococcus between normal and clinically affected animals. Cultures of 135 repeat samples from 33 cows revealed that ureaplasma persisted in some animals for at least three months. No viruses were isolated from any of the animals in this study.
从牛外阴拭子中培养支原体、病毒和细菌，以确定脲原体是否与安大略省16个奶牛场观察到的临床颗粒性外阴炎有关。从34头临床正常奶牛中的23.5%、27头有轻度至中度外阴充血但无分泌物的奶牛中的74%以及20头伴有脓性分泌物的急性外阴充血奶牛中的100%分离出脲原体。临床组之间的分离率存在统计学显著差异。分别从患有中度或急性外阴炎的奶牛中的7.7%和20%分离出牛生殖支原体，但未从正常奶牛中分离出。从25%患有急性外阴炎的奶牛中分离出睡眠嗜血杆菌。正常动物和临床受影响动物之间在大肠杆菌、化脓性棒状杆菌和α溶血性链球菌的分离方面没有显著差异。对33头奶牛的135份重复样本进行培养，结果显示脲原体在一些动物中持续存在至少三个月。本研究中未从任何动物中分离出病毒。
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