Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Digestive Oncology Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
Nucleosides Nucleotides Nucleic Acids. 2022;41(4):370-383. doi: 10.1080/15257770.2022.2046278. Epub 2022 Mar 6.
Exhaled breath condensate (EBC) is used to investigate the efficacy of EBC to detect the genetic mutations in patients with lung cancer. Samples of 5 patients and 5 healthy volunteers were collected. DNA was extracted and used for amplification of hotspot regions of and genes by using PCR. We performed the mutation analysis by direct sequencing in all subjects. Detected mutations in EBC samples were compared with those of corresponding tumor tissues and there was complete agreement within the detected mutations in EBC and tumorous tissue. EBC can be used as an efficient and noninvasive source for the assessment of gene mutations in patients with lung cancer.
呼出气冷凝物（EBC）用于研究 EBC 检测肺癌患者基因突变的效果。收集了 5 名患者和 5 名健康志愿者的样本。提取 DNA，通过 PCR 扩增 和 基因的热点区域。对所有受试者进行直接测序进行突变分析。比较 EBC 样本中的检测突变与相应肿瘤组织中的突变，EBC 和肿瘤组织中的检测突变完全一致。EBC 可作为评估肺癌患者基因突变的有效、非侵入性来源。
