Paramasivam M
Pesticide Toxicology Laboratory, Department of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu 641003 India.
J Food Sci Technol. 2022 Apr;59(4):1549-1557. doi: 10.1007/s13197-021-05165-7. Epub 2021 Jun 4.
A multiresidue method was developed and validated for the simultaneous determination of eight synthetic pyrethroids and hexaconazole fungicide residues in curry leaves. The method involves extraction of leaf sample with ethyl acetate, purified by dispersive SPE with primary secondary amine and graphitized carbon black. The gas chromatography-electron capture detector (GC-ECD) used for residue estimation and confirmed using gas chromatography-mass spectrometry (GC-MS) with the LOQ of 0.03 μg/g. The recoveries of all pesticides were in the range of 75 to 110% with a relative standard deviation of less than 7%. The matrix effect was within the acceptable limit of less than 20% as prescribed by SANTE guidelines. The decontamination was carried out to reduce the concentration of residues in curry leaves by different household techniques and lemon juice (2%) solution was found to be more effective (35.71-56.52%) followed by 2% tamarind (23.48 to 42.94%), hot water (18.44 to 46.74%), 2% salt water (10.98 to 42.71%) and least in tap water (3.57 to 27.84%). The method was successfully applied for routine monitoring of pesticide residues in curry leaves.
建立并验证了一种多残留方法，用于同时测定咖喱叶中8种拟除虫菊酯类农药和己唑醇杀菌剂残留量。该方法包括用乙酸乙酯提取叶片样品，并用伯仲胺和石墨化炭黑通过分散固相萃取进行净化。使用气相色谱 - 电子捕获检测器（GC - ECD）进行残留量测定，并通过气相色谱 - 质谱联用仪（GC - MS）进行确证，定量限为0.03μg/g。所有农药的回收率在75%至110%之间，相对标准偏差小于7%。基质效应在SANTE指南规定的小于20%的可接受限度内。采用不同的家庭技术对咖喱叶进行去污处理以降低残留浓度，发现2%柠檬汁溶液效果更佳（35.71 - 56.52%），其次是2%罗望子溶液（23.48至42.94%）、热水（18.44至46.74%）、2%盐水（10.98至42.71%），而自来水效果最差（3.57至27.84%）。该方法成功应用于咖喱叶中农药残留的常规监测。
