Pesticide Toxicology Laboratory, Department of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore 03, Tamil Nadu, India.
Food Chem. 2020 Aug 15;321:126678. doi: 10.1016/j.foodchem.2020.126678. Epub 2020 Mar 23.
A simple analytical method was developed and validated, as per SANTE 2017 guidelines, for simultaneous determination of 10 organophosphorus residues in curry leaf using gas chromatography with flame photometric detection, and confirmed using gas chromatography-mass spectrometry. Samples were extracted with ethyl acetate and cleaned up with primary secondary amine and graphitized carbon black. Average recoveries were in the range 80-108% with the RSD of less than 8%. The effects of different household cleaning techniques to reduce the concentration of pesticide residues in curry leaf were evaluated and found to remove 2-65% of residues. The method was applied to analyse sample to fresh curry leaf samples, and analysis of potential health risks estimated the residues did not pose a direct hazard. This method could be useful for routine analysis of organophosphorus residue for monitoring purposes.
本研究按照 SANTE 2017 指南，建立并验证了一种可同时检测咖喱叶中 10 种有机磷残留的分析方法，该方法采用气相色谱-火焰光度检测法，并通过气相色谱-质谱法进行确证。样品采用乙酸乙酯提取，经伯仲胺和石墨化炭黑净化。平均回收率为 80-108%，相对标准偏差（RSD）小于 8%。评估了不同家庭清洁技术对降低咖喱叶中农药残留浓度的影响，结果发现可去除 2-65%的残留。该方法应用于新鲜咖喱叶样品的分析，对潜在健康风险的分析表明残留不会造成直接危害。本方法可用于有机磷残留的常规分析监测。
