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用于从颊细胞获取基因组DNA的微针拭子的开发。

Development of a Microneedle Swab for Acquisition of Genomic DNA From Buccal Cells.

作者信息

Kim Yun-Seo, Kim JeongHyeon, Na Woonsung, Sung Gil-Hwan, Baek Seung-Ki, Kim Yun Kyoung, Kim Gyeong Ryeong, Hu Hae-Jin, Park Jung-Hwan

机构信息

Department of Bionano Technology and Gachon BioNano Research Institute, Gachon University, Seongnam, South Korea.

Laboratory of Veterinary Virology, College of Veterinary Medicine, Chonnam National University, Gwangju, South Korea.

出版信息

Front Bioeng Biotechnol. 2022 Feb 18;10:829648. doi: 10.3389/fbioe.2022.829648. eCollection 2022.

DOI:10.3389/fbioe.2022.829648
PMID:35252137
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8895340/
Abstract

A swab is a tool for obtaining buccal DNA from buccal mucus for biological analysis. The acquisition of a sufficient amount and high quality of DNA is an important factor in determining the accuracy of a diagnosis. A microneedle swab (MN swab) was developed to obtain more oral mucosal tissues non-invasively. Eight types of MN swabs were prepared with varying combinations of patterns (zigzag or straight), number of MNs, intervals of MNs, and sharpness of tips. When MN swab was applied up to 10 times, the tissue amount and DNA yield increased compared to commercial swabs. A zigzag pattern of microneedles was found to be more efficient than a straight pattern and increasing the number of microneedles in an array increased the DNA yield. The MN swab collected about twice the DNA compared to the commercial swab. In an test using mini pigs, the lower cycle threshold values of mucosal samples collected with MN swabs compared to samples collected with commercial swabs indicated that a greater amount of DNA was collected for SNP genotyping. A polymer MN swab is easy to manufacture by a single molding process, and it has a greater sampling capacity than existing commercial swabs.

摘要

拭子是一种从颊黏膜获取颊部DNA用于生物学分析的工具。获取足量且高质量的DNA是决定诊断准确性的重要因素。已研发出一种微针拭子（MN拭子），用于无创获取更多口腔黏膜组织。制备了八种微针拭子，其图案（之字形或直线形）、微针数量、微针间距和针尖锐度组合各不相同。与商用拭子相比，微针拭子应用多达10次时，组织量和DNA产量会增加。发现微针的之字形图案比直线形图案更有效，且增加阵列中的微针数量会提高DNA产量。微针拭子收集的DNA量约为商用拭子的两倍。在一项使用小型猪的测试中，与用商用拭子收集的样本相比，用微针拭子收集的黏膜样本的较低循环阈值表明，为单核苷酸多态性基因分型收集到了更多的DNA。聚合物微针拭子易于通过单一成型工艺制造，且其采样能力比现有的商用拭子更强。

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Safe Coated Microneedles with Reduced Puncture Occurrence after Administration.给药后穿刺发生率降低的安全涂层微针。
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Clinical Utility of Buccal Swabs for Severe Acute Respiratory Syndrome Coronavirus 2 Detection in Coronavirus Disease 2019-Infected Children.
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The influence of a swab type on the results of point-of-care tests.拭子类型对即时检测结果的影响。
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