Themmen A P, Hoogerbrugge J W, Rommerts F F, van der Molen H J
FEBS Lett. 1986 Jul 28;203(2):116-20. doi: 10.1016/0014-5793(86)80725-6.
We have studied the possible involvement of the activation of calcium-dependent phospholipid-activated protein kinase (PK-C) in the stimulatory action of LHRH on Leydig cells, using 4 beta-phorbol-12-myristate-13-acetate (PMA) and phospholipase C (PL-C). LHRH agonist (LHRH-A) and PL-C had a large synergistic effect on LH-stimulated steroid production, whereas PMA inhibited the effect of LH. However, PMA always caused an increase in steroid production stimulated by various doses of dibutyryl cAMP. LH and PMA stimulated the phosphorylation of 17 and 33 kDa proteins, whereas LHRH-A and PL-C had no effect. Of all effectors used, LH had the most pronounced effect on the synthesis of 14, 27 and 30 kDa proteins. The present results suggest that the mechanisms of action of LHRH-A and PL-C on steroid production in Leydig cells may be similar and different from PMA, and may involve stimulation of a specific type of PK-C or hydrolysis of a specific pool of phospholipids.
我们使用4β-佛波醇-12-肉豆蔻酸酯-13-乙酸酯（PMA）和磷脂酶C（PL-C），研究了钙依赖性磷脂激活蛋白激酶（PK-C）的激活在促性腺激素释放激素（LHRH）对睾丸间质细胞的刺激作用中可能的参与情况。LHRH激动剂（LHRH-A）和PL-C对促黄体生成素（LH）刺激的类固醇生成具有很大的协同作用，而PMA则抑制LH的作用。然而，PMA总是导致不同剂量的二丁酰环磷腺苷（dbcAMP）刺激的类固醇生成增加。LH和PMA刺激了17 kDa和33 kDa蛋白质的磷酸化，而LHRH-A和PL-C则没有影响。在所使用的所有效应物中，LH对14 kDa、27 kDa和30 kDa蛋白质的合成影响最为显著。目前的结果表明，LHRH-A和PL-C对睾丸间质细胞类固醇生成的作用机制可能与PMA相似且不同，可能涉及刺激特定类型的PK-C或水解特定池的磷脂。
