University of Illinois at Urbana-Champaign, Urbana, IL.
Independant Consultant, Columbia, TN.
Vet Pathol. 2022 May;59(3):427-432. doi: 10.1177/03009858221083035. Epub 2022 Mar 6.
In dogs, primary bone tumors can be difficult to distinguish with histopathology. Of those tumors, osteosarcoma (OSA) is the most common and aggressive. In this study, 4 immunohistochemistry markers-alkaline phosphatase (ALP), osteonectin (ON), osteopontin (OP), and runx2-were evaluated for their ability to distinguish OSA from other primary bone tumors. The 42 formalin-fixed, paraffin-embedded, primary canine bone tumors included 15 OSAs, 8 chondrosarcomas, 11 fibrosarcomas, and 8 histiocytic sarcomas. All 4 antibodies were highly sensitive for detection of osteosarcoma. ALP was the most sensitive at 100% and runx2 the most specific at 78%. Running ALP and runx2 in series resulted in a sensitivity of 87% and a specificity of 85%. This combination of immunomarkers resulted in a diagnostic panel for distinguishing osteosarcoma from other primary bone tumors.
在狗中，原发性骨肿瘤在组织病理学上可能难以区分。在这些肿瘤中，骨肉瘤（OSA）最为常见且具有侵袭性。在这项研究中，评估了 4 种免疫组织化学标志物——碱性磷酸酶（ALP）、骨连接蛋白（ON）、骨桥蛋白（OP）和 runt 相关转录因子 2（runx2）——用于区分 OSA 与其他原发性骨肿瘤的能力。42 例福尔马林固定、石蜡包埋的原发性犬骨肿瘤包括 15 例骨肉瘤、8 例软骨肉瘤、11 例纤维肉瘤和 8 例组织细胞肉瘤。所有 4 种抗体对骨肉瘤的检测均具有很高的敏感性。ALP 的敏感性最高，为 100%，runx2 的特异性最高，为 78%。ALP 和 runx2 串联运行的敏感性为 87%，特异性为 85%。这种免疫标志物组合可用于区分骨肉瘤和其他原发性骨肿瘤的诊断面板。
