Inoue M, Delellis R A, Scully R E
Hum Pathol. 1986 Aug;17(8):841-7. doi: 10.1016/s0046-8177(86)80205-2.
Forty-five endometrial carcinomas, 36 of which contained argyrophil cells and nine of which were nonargyrophilic by the Grimelius method, were examined immunohistochemically for chromogranin. Chromogranin immunoreactivity was present in 19 of the 36 tumors with argyrophil cells (53 per cent) and in none of the nine tumors lacking these cells. All six of the tumors that contained argyrophil cells resembling enterochromaffin cells were chromogranin-positive, with the staining corresponding to the argyrophilia. In contrast, only 13 of the 30 tumors in which argyrophilia was present in the apical region or throughout the cytoplasm of the cells showed chromogranin immunoreactivity. In seven of these tumors, an excellent correlation existed between the distribution of argyrophilia and chromogranin positivity, but in the other six tumors argyrophilia was more pronounced than chromogranin immunoreactivity. Adjacent to one tumor, unusual cells in which argyrophil granules were packed predominantly in the basal portion of the cytoplasm were encountered in a focus of atypical hyperplasia; these cells were also chromogranin-positive. The present observations suggest that endometrial carcinoma cells with diffuse or apical chromogranin immunoreactivity may represent an early stage in the development of cells resembling those of the enterochromaffin type.
对45例子宫内膜癌进行嗜铬粒蛋白免疫组织化学检测，其中36例用Grimelius法检测到嗜银细胞，9例未检测到嗜银细胞。在36例有嗜银细胞的肿瘤中，19例（53%）呈嗜铬粒蛋白免疫反应阳性，而9例无嗜银细胞的肿瘤均为阴性。所有6例含有类似肠嗜铬细胞的嗜银细胞的肿瘤均为嗜铬粒蛋白阳性，染色与嗜银性相对应。相比之下，在30例细胞顶端区域或整个细胞质存在嗜银性的肿瘤中，只有13例呈嗜铬粒蛋白免疫反应阳性。在其中7例肿瘤中，嗜银性分布与嗜铬粒蛋白阳性之间存在良好的相关性，但在其他6例肿瘤中，嗜银性比嗜铬粒蛋白免疫反应更明显。在一个肿瘤旁的非典型增生灶中，发现异常细胞，其嗜银颗粒主要聚集在细胞质的基部；这些细胞也呈嗜铬粒蛋白阳性。目前的观察结果表明，具有弥漫性或顶端嗜铬粒蛋白免疫反应的子宫内膜癌细胞可能代表了类似肠嗜铬细胞类型细胞发育的早期阶段。
