Ueda G, Shimizu C, Saito J, Inoue Y, Tanaka Y, Inoue M, Tanizawa O
Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
Int J Gynaecol Obstet. 1989 Jun;29(2):165-72. doi: 10.1016/0020-7292(89)90848-5.
Various gynecologic tumors with argyrophilia were studied immunohistochemically for chromogranin using two antibodies, antichromogranin and antineuroendocrine. Of seven small cell carcinomas of the cervix, four were immunoreactive with antichromogranin and seven with antineuroendocrine. Argyrophil cells of six cervical adenocarcinomas were all immunoractive with both antibodies. Type I argyrophil cells of 20 endometrial carcinomas were likewise stained positively. However, of the 30 endometrial carcinomas with type II argyrophil cells, 19 showed positive immunoreactivity for chromogranin and 22 for neuroendocrine. Of the ovarian tumors tested, argyrophil cells of 11 mucinous tumors, three carcinoid tumors, and the pancreatic tissue of a malignant mixed germ cell tumor were all chromogranin- and neuroendocrine-immunoreactive. Type I argyrophil cells of five endometrioid carcinomas of the ovary were also immunoreactive with both antibodies. Of the 13 endometrioid carcinomas with type II argyrophil cells, only four showed positive immunoreactivity for chromogranin and only five for neuroendocrine. In conclusion, both antichromogranin and antineuroendocrine detect the specific neuroendocrine markers in close association with argyrophilia in gynecologic tumors, the latter being more sensitive for small cell carcinoma of the cervix, and for type II argyrophil cells in adenocarcinoma of the endometrium and endometrioid carcinoma of the ovary.
使用抗嗜铬粒蛋白和抗神经内分泌这两种抗体，对各种嗜银性妇科肿瘤进行嗜铬粒蛋白免疫组化研究。在7例宫颈小细胞癌中，4例对抗嗜铬粒蛋白呈免疫反应，7例对抗神经内分泌呈免疫反应。6例宫颈腺癌的嗜银细胞对两种抗体均呈免疫反应。20例子宫内膜癌的Ⅰ型嗜银细胞同样呈阳性染色。然而，在30例具有Ⅱ型嗜银细胞的子宫内膜癌中，19例对嗜铬粒蛋白呈阳性免疫反应，22例对神经内分泌呈阳性免疫反应。在所检测的卵巢肿瘤中，11例黏液性肿瘤、3例类癌肿瘤以及1例恶性混合性生殖细胞肿瘤的胰腺组织中的嗜银细胞均对嗜铬粒蛋白和神经内分泌呈免疫反应。5例卵巢子宫内膜样癌的Ⅰ型嗜银细胞对两种抗体也呈免疫反应。在13例具有Ⅱ型嗜银细胞的子宫内膜样癌中，仅4例对嗜铬粒蛋白呈阳性免疫反应，仅5例对神经内分泌呈阳性免疫反应。总之，抗嗜铬粒蛋白和抗神经内分泌均能检测出与妇科肿瘤嗜银性密切相关的特异性神经内分泌标志物，后者对宫颈小细胞癌、子宫内膜腺癌和卵巢子宫内膜样癌中的Ⅱ型嗜银细胞更为敏感。
