Sorbonne Université, CNRS, Laboratoire de Biologie du Développement - Institut de Biologie Paris Seine (LBD-IBPS), Cell cycle and cell determination Team, Paris, France.
Sorbonne Université, INSERM, NutriOmics Research Unit, Paris, France.
Elife. 2022 Mar 7;11:e75746. doi: 10.7554/eLife.75746.
Spatiotemporal mechanisms generating neural diversity are fundamental for understanding neural processes. Here, we investigated how neural diversity arises from neurons coming from identical progenitors. In the dorsal thorax of , rows of mechanosensory organs originate from the division of sensory organ progenitor (SOPs). We show that in each row of the notum, an anteromedial located central SOP divides first, then neighbouring SOPs divide, and so on. This centrifugal wave of mitoses depends on cell-cell inhibitory interactions mediated by SOP cytoplasmic protrusions and Scabrous, a secreted protein interacting with the Delta/Notch complex. Furthermore, when this mitotic wave was reduced, axonal growth was more synchronous, axonal terminals had a complex branching pattern and fly behaviour was impaired. We show that the temporal order of progenitor divisions influences the birth order of sensory neurons, axon branching and impact on grooming behaviour. These data support the idea that developmental timing controls axon wiring neural diversity.
产生神经多样性的时空机制对于理解神经过程至关重要。在这里，我们研究了来自相同祖细胞的神经元如何产生神经多样性。在 的背部胸腔中，一排排机械感受器器官起源于感觉器官祖细胞（SOP）的分裂。我们发现，在背板的每一行中，位于前内侧的中央 SOP 首先分裂，然后相邻的 SOP 分裂，依此类推。这种有丝分裂的离心波取决于 SOP 细胞质突起和 Scabrous 介导的细胞间抑制相互作用，Scabrous 是一种与 Delta/Notch 复合物相互作用的分泌蛋白。此外，当这种有丝分裂波减少时，轴突生长更加同步，轴突末端具有复杂的分支模式，并且飞行行为受损。我们表明，祖细胞分裂的时间顺序会影响感觉神经元的出生顺序、轴突分支和对梳理行为的影响。这些数据支持了这样一种观点，即发育时间控制着轴突连接的神经多样性。
