Biochemistry and Genetics of Microorganisms (BGM), Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Croix du Sud 4-5, (box L7.07.06), B-1348 Louvain-la-Neuve, Belgium.
Health and Biosciences, IFF Danisco France SAS, CS 10010, F-86220 Dangé-Saint-Romain, France.
FEMS Microbiol Rev. 2022 Jul 20;46(4). doi: 10.1093/femsre/fuac014.
Nowadays, the growing human population exacerbates the need for sustainable resources. Inspiration and achievements in nutrient production or human/animal health might emanate from microorganisms and their adaptive strategies. Here, we exemplify the benefits of lactic acid bacteria (LAB) for numerous biotechnological applications and showcase their natural transformability as a fast and robust method to hereditarily influence their phenotype/traits in fundamental and applied research contexts. We described the biogenesis of the transformation machinery and we analyzed the genome of hundreds of LAB strains exploitable for human needs to predict their transformation capabilities. Finally, we provide a stepwise rational path to stimulate and optimize natural transformation with standard and synthetic biology techniques. A comprehensive understanding of the molecular mechanisms driving natural transformation will facilitate and accelerate the improvement of bacteria with properties that serve broad societal interests.
如今，不断增长的人口加剧了对可持续资源的需求。营养生产或人类/动物健康方面的灵感和成就可能源自微生物及其适应性策略。在这里，我们举例说明了乳酸菌 (LAB) 在众多生物技术应用中的益处，并展示了它们作为一种快速而强大的方法的自然可变性，可在基础和应用研究环境中遗传地影响其表型/特征。我们描述了转化机制的生物发生，并分析了可用于满足人类需求的数百种 LAB 菌株的基因组，以预测它们的转化能力。最后，我们提供了一条逐步的合理路径，用标准和合成生物学技术来刺激和优化自然转化。对驱动自然转化的分子机制的全面理解将有助于并加速改善具有广泛社会利益的细菌。
