Behrens P Q, Nakashima H, Yokota E, Riggs A F
J Biol Chem. 1986 Aug 15;261(23):10520-5.
Fourteen fragments have been isolated from hemocyanin component II of Limulus polyphemus by cleavage with CNBr. The amino acid sequence of the largest fragment, CNBr Ia has been reported (Yokota, E., and Riggs, A. F. (1984) J. Biol. Chem. 259, 4739-4749). The amino acid sequence of the 12 smaller fragments is reported in an accompanying paper (Moore, M. D., Behrens, P. Q., and Riggs, A. F. (1985) J. Biol. Chem. 261, 10511-10519). We have determined the amino acid sequence of the second largest fragment, CNBr Ib. The fragment contains 142 residues and has a molecular weight of 16,095.
通过用溴化氰切割，从鲎血蓝蛋白组分II中分离出了14个片段。最大的片段CNBr Ia的氨基酸序列已有报道（横田英、里格斯AF（1984年）《生物化学杂志》259卷，4739 - 4749页）。12个较小片段的氨基酸序列在随附论文中报道（摩尔MD、贝伦斯PQ、里格斯AF（1985年）《生物化学杂志》261卷，10511 - 10519页）。我们已经确定了第二大的片段CNBr Ib的氨基酸序列。该片段包含142个残基，分子量为16,095。
