Nakashima H, Behrens P Q, Moore M D, Yokota E, Riggs A F
J Biol Chem. 1986 Aug 15;261(23):10526-33.
The amino acid sequence of the largest fragment, CNBr Ia (203 residues) has been reported (Yokota, E., and Riggs, A. F. (1984) J. Biol. Chem. 259, 4739-4749). The amino acid sequences of the second largest fragment, CNBr Ib (142 residues), and of the 12 smaller fragments are reported in accompanying papers (Moore, M. D., Behrens, P. Q., and Riggs, A. F. (1986) J. Biol. Chem. 261, 10511-10519; Behrens, P. Q., Nakashima, H., and Riggs, A. F. (1986) J. Biol. Chem. 261, 10520-10525). The complete amino acid sequence of hemocyanin component II has been established by isolation and analysis of 13 methionine-containing peptides from either a tryptic digest or a Staphylococcus aureus strain V8 protease digest of whole carboxamidomethylated hemocyanin II. Hemocyanin II is composed of 628 residues and has a molecular weight with two copper atoms of 72,946.
最大片段CNBr Ia（203个残基）的氨基酸序列已见报道（横田英和里格斯AF（1984年）《生物化学杂志》259卷，4739 - 4749页）。第二大片段CNBr Ib（142个残基）以及12个较小片段的氨基酸序列见随附论文（摩尔MD、贝伦斯PQ和里格斯AF（1986年）《生物化学杂志》261卷，10511 - 10519页；贝伦斯PQ、中岛H和里格斯AF（1986年）《生物化学杂志》261卷，10520 - 10525页）。血蓝蛋白组分II的完整氨基酸序列是通过从全羧甲基化血蓝蛋白II的胰蛋白酶消化物或金黄色葡萄球菌V8蛋白酶消化物中分离并分析13个含甲硫氨酸的肽段确定的。血蓝蛋白II由628个残基组成，含两个铜原子时分子量为72,946。
