Rumen fluke infections (Paramphistomidae) in diarrhoeal cattle in western France and association with production parameters.

This study was conducted to assess the impact of rumen flukes (RFs) (Paramphistomatidae) on various production parameters of cattle in Normandy. Faecal and blood samples were taken between 1 January 2010 and 31 December 2019 as part of the routine diagnostic activity for diarrhoea in weaned cattle, including a quantitative parasitological analysis coupled with a search for Johne's disease (paratuberculosis). Information on slaughter and carcass weight, inter-farm movements and mortality was obtained from the French national registration database (BDNI). The study was conducted at two levels using adapted categorical RF variables: 1) at the cattle level (>12 months), an estimation of presence of adult parasites using egg count in faecal samples (negative vs. positive) and 2) at the herd level, an estimation of 6-24 months of the dairy heifers exposure to larval forms on the basis of the proportion of dairy cattle shedding eggs in the herd (three classes). At the cattle level, the outcome variables were carcass weight (model 1), case-control status for mortality within 30 days of diagnosis (model 2) and case-control status for slaughter within 365 days of diagnosis (model 3). At the herd level, the outcome variable was the mortality ratio for dairy heifers aged 6-24 months (Model 4). Other important covariates were used to improve model fit. Multivariate analyses were performed using a linear mixed model (model 1), generalised estimating equations (GEE) (models 2 and 3) and a multinomial logistic model (model 4). Overall, 1291 out of 4315 cattle (29.9%) were found to excrete RF eggs and 19.6% of the positives had an excretion score of >200 eggs/g. The prevalence increased steadily with age class and was higher in beef cattle than dairy cattle (42.7% vs. 26.9%) in the years 2015-2019 compared to 2010-2014 (33.3% vs. 26.5%) and in November-February (33.2%) compared to March-June (28.9%) and July-October (27.3%). Rumen fluke variables were not found to be explanatory factors of outcome variables at both animal and herd levels. In contrast, significant negative associations were observed between outcome variables and other health covariates, such as Johne's disease, GI nematode, bovine viral diarrhoea and coccidia statuses. In conclusion, RFs are prevalent in cattle reared in Normandy but this does not result in significant production losses. Therefore, the value to farmers of oxyclozanide treatment at an effective dose for paramphistomosis after simple identification of RF eggs in the faeces seems limited.