Department of Pediatrics, Section of Diabetes/Endocrinology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.
Department of Pediatrics, Section of Diabetes/Endocrinology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.
Nutr Metab Cardiovasc Dis. 2022 May;32(5):1253-1258. doi: 10.1016/j.numecd.2022.01.034. Epub 2022 Feb 10.
Soluble adhesion molecules are associated with cardiovascular disease and increased in individuals with diabetes. This study assesses the impact of diabetes exposure in utero on the abundance of circulating adhesion molecules in cord serum and soluble adhesion molecules released from human umbilical vein endothelial cells (HUVEC) exposed to high glucose concentrations.
Women with and without diabetes were recruited. DM was diagnosed based on the American Diabetes Association criteria. Primary cultures of HUVEC were cultured in 5 mM and 25 mM glucose with 25 mM mannitol osmotic control. The soluble adhesion molecules, intracellular adhesion molecule (ICAM), vascular cell adhesion molecule (VCAM) and E-selectin were measured by ELISA in the cord blood serum and conditioned HUVEC media. The mothers with DM were older with higher BMI (p = 0.027 and 0.008, respectively). In a fully adjusted model, VCAM was significantly increased in the cord serum of infants born to mothers with diabetes (p = 0.046), but ICAM and E-selectin were not different. ICAM was also significantly correlated with maternal HbA1c (r = 0.16, p = 0.004) and cord serum non-esterified fatty acids (r = 0.08, p = 0.013). From the HUVEC media, the abundance of adhesion molecules was not different based on DM or high glucose exposure; however, VCAM abundance in the HUVEC supernatant was significantly correlated with ICAM (r = 0.27, p = 0.010) and cord serum c-peptide (R = 0.19, p = 0.043).
Alterations in soluble adhesion molecule abundance in infants exposed to the diabetic milieu of pregnancy may reflect early alterations in vascular function predicting future cardiovascular disease.
可溶性黏附分子与心血管疾病相关，且在糖尿病患者中增加。本研究评估了宫内暴露于糖尿病对脐血清中循环黏附分子丰度的影响，以及暴露于高葡萄糖浓度下人脐静脉内皮细胞（HUVEC）释放的可溶性黏附分子。
招募了患有糖尿病和不患有糖尿病的女性。根据美国糖尿病协会的标准诊断为 DM。将 HUVEC 原代培养物分别在 5mM 和 25mM 葡萄糖与 25mM 甘露醇渗透对照下培养。通过 ELISA 在脐血血清和条件化 HUVEC 培养基中测量可溶性黏附分子细胞间黏附分子（ICAM）、血管细胞黏附分子（VCAM）和 E-选择素。患有 DM 的母亲年龄较大，BMI 较高（p=0.027 和 0.008）。在完全调整模型中，出生于糖尿病母亲的婴儿脐血清中 VCAM 显著增加（p=0.046），但 ICAM 和 E-选择素无差异。ICAM 与母亲的 HbA1c 也呈显著正相关（r=0.16，p=0.004）和脐血清非酯化脂肪酸（r=0.08，p=0.013）。从 HUVEC 培养基中，DM 或高葡萄糖暴露的黏附分子丰度没有差异；然而，HUVEC 上清液中 VCAM 的丰度与 ICAM 呈显著正相关（r=0.27，p=0.010）和脐血清 C 肽（R=0.19，p=0.043）。
在妊娠糖尿病环境中暴露的婴儿中可溶性黏附分子丰度的改变可能反映了血管功能的早期改变，预测未来的心血管疾病。
