Soares Vanessa Mendonça, Dos Santos Emanoelli Aparecida Rodrigues, Tadielo Leonardo Ereno, Cerqueira-Cézar Camila Koutsodontis, da Cruz Encide Sampaio Aryele Nunes, Eisen Ana Karolina Antunes, de Oliveira Kelen Gras, Padilha Matheus Beltrame, de Moraes Guerra Maria Eduarda, Gasparetto Raíssa, Brum Mário Celso Sperotto, Traesel Carolina Kist, Henzel Andreia, Spilki Fernando Rosado, Pereira Juliano Gonçalves
Universidade Federal do Pampa, Campus Uruguaiana, BR 472, Km 585, Uruguaiana, Rio Grande do Sul CEP 97501970, Brazil.
Universidade Federal do Paraná, Setor Palotina, Rua Pioneiro, 2153, Palotina, Paraná CEP 85950000, Brazil.
One Health. 2022 Mar 1;14:100377. doi: 10.1016/j.onehlt.2022.100377. eCollection 2022 Jun.
The aim of this study was to investigate the presence of adenovirus (AdV), rotavirus (RV), and hepatitis E virus (HEV) in beef, pork, and chicken meat cuts in retail trade in the city of Uruguaiana, RS, Brazil. A total of 131 meat products were collected (beef, = 55; chicken, = 47; pork, = 29) from 18 commercial establishments (supermarkets, = 7; butchers, = 7; markets/grocery stores, n = 4). All samples were evaluated for AdV, RV, and HEV. The genomes of RV and AdV were identified in 29% ( = 38) and 5.34% ( = 7) of the samples, respectively. HEV was not identified in any of the samples. Chicken cuts had a higher frequency of AdV and RV isolates compared to beef and pork ( < 0.05). Among the categories of commercial establishments evaluated, all revealed at least one positive sample for AdV and RV; however, supermarkets showed a higher frequency of RV than others ( < 0.05). The genetic material of AdV and RV was identified simultaneously in 2.29% ( = 3) of samples from supermarkets ( = 2) and grocery stores ( = 1). This is the first report on detection of enteric viruses in meat cuts in the western region of the state of Rio Grande do Sul, Brazil, and the presence of AdV and RV in these products may indicate flaws during the process of handling these foods, especially in places where commercialization provides important public health issues.
本研究旨在调查巴西南里奥格兰德州乌鲁瓜亚纳市零售贸易中的牛肉、猪肉和鸡肉切块中是否存在腺病毒(AdV)、轮状病毒(RV)和戊型肝炎病毒(HEV)。从18家商业机构(超市7家、肉店7家、市场/杂货店4家)共采集了131份肉类产品(牛肉55份、鸡肉47份、猪肉29份)。所有样本均检测AdV、RV和HEV。分别在29%(n = 38)和5.34%(n = 7)的样本中鉴定出RV和AdV的基因组。所有样本中均未鉴定出HEV。与牛肉和猪肉相比,鸡肉切块中AdV和RV分离株的频率更高(P < 0.05)。在所评估的商业机构类别中,所有机构均至少有一个AdV和RV阳性样本;然而,超市中RV的频率高于其他机构(P < 0.05)。在来自超市(n = 2)和杂货店(n = 1)的2.29%(n = 3)的样本中同时鉴定出AdV和RV的遗传物质。这是巴西南里奥格兰德州西部地区肉类切块中肠道病毒检测的首份报告,这些产品中AdV和RV的存在可能表明在这些食品的处理过程中存在缺陷,尤其是在商业化带来重要公共卫生问题的地方。