Borghi Monica, Pierboni Elisa, Primavilla Sara, Scoccia Eleonora, Costantini Claudio, Suffredini Elisabetta, Graziani Alessandro, Macellari Piero, Macrì Salvatore, Farneti Silvana, Valiani Andrea
Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche "T. Rosati", 06126 Perugia, Italy.
Department of Medicine and Surgery, Pathology Division, University of Perugia, L. Severi Square, 06129 Perugia, Italy.
Foods. 2024 Aug 9;13(16):2504. doi: 10.3390/foods13162504.
Consumption of raw or undercooked wild boar (WB) meat is considered an important risk factor for hepatitis E virus (HEV) infection in humans. The possibility of HEV contamination during the slaughtering practices may pose an additional risk. Based on these assumptions, we evaluated HEV contamination of WB meat hunted in Umbria (central Italy) during the 2022-2023 hunting season by real-time RT-PCR. Herein, we show that 10.8% of livers from slaughtered WB were positive for HEV RNA, thus providing an estimate of HEV infection in WB in the Umbria region. Then, by evaluating paired liver-muscle samples from both HEV-positive and HEV-negative animals, we found evidence of muscle HEV contamination in 33% and 14% of cases, respectively. This is the first report on the detection of HEV in WB meat in Umbria, an Italian region with diffuse WB hunting and consumption. The evidence of contamination provided by our study underscores the importance of adopting good hygienic practices in the processing stages of hunted WB carcasses to significantly reduce meat contamination and the risk posed for the final consumer.
食用生的或未煮熟的野猪（WB）肉被认为是人类感染戊型肝炎病毒（HEV）的一个重要风险因素。屠宰过程中戊型肝炎病毒污染的可能性可能会带来额外风险。基于这些假设，我们通过实时逆转录聚合酶链反应（RT-PCR）评估了2022 - 2023年狩猎季节在翁布里亚（意大利中部）猎获的野猪肉中的戊型肝炎病毒污染情况。在此，我们表明，屠宰的野猪肝脏中10.8%的戊型肝炎病毒核糖核酸（HEV RNA）呈阳性，从而提供了翁布里亚地区野猪戊型肝炎病毒感染的一个估计值。然后，通过评估戊型肝炎病毒阳性和阴性动物的肝脏 - 肌肉配对样本，我们分别在33%和14%的病例中发现了肌肉戊型肝炎病毒污染的证据。这是关于在翁布里亚（一个野猪狩猎和消费普遍的意大利地区）的野猪肉中检测到戊型肝炎病毒的首次报告。我们的研究提供的污染证据强调了在狩猎的野猪 carcasses加工阶段采用良好卫生习惯以显著减少肉类污染和给最终消费者带来的风险的重要性。
