斑纹电鳐胆碱能电动系统中血管活性肠肽样免疫反应性的存在。

Presence of vasoactive intestinal polypeptide-like immunoreactivity in the cholinergic electromotor system of Torpedo marmorata.

作者信息

Agoston D V, Conlon J M

出版信息

J Neurochem. 1986 Aug;47(2):445-53. doi: 10.1111/j.1471-4159.1986.tb04521.x.

DOI:10.1111/j.1471-4159.1986.tb04521.x

Abstract

Vasoactive intestinal polypeptide (VIP)-like immunoreactivity was detected in the cholinergic electro-motor system of Torpedo marmorata using a combination of immunohistochemical assays, radioimmunoassay, and HPLC. The immunohistochemical assays revealed that the distribution of VIP-like immunoreactivity in the electric lobes, electromotor nerves, and electric organ is comparable to that of the stable cholinergic synaptic vesicle marker vesicle-specific proteoglycan. Ligation of the electromotor nerves caused a marked accumulation of VIP-like immunoreactivity in the lobes (180%) and the proximal portions of the electromotor nerves (130%) and a decrease in the electric organ (-50%), when measured by radioimmunoassay using synthetic VIP (porcine sequence) as the standard. VIP-like immunoreactivity in extracts of electric lobes electromotor nerves, and electric organ was eluted from a semipreparative reverse-phase HPLC column as a single peak with a retention time similar to that of porcine VIP. Rechromatography at higher resolution on an analytical column indicated diversity between the molecular forms of VIP-like immunoreactivity extracted from electric lobe and electric organ, suggesting the possibility of posttranslational processing.

摘要

运用免疫组织化学分析、放射免疫分析和高效液相色谱法相结合的方法，在斑纹电鳐的胆碱能电动系统中检测到了血管活性肠肽（VIP）样免疫反应性。免疫组织化学分析显示，VIP样免疫反应性在电叶、电动神经和电器官中的分布与稳定的胆碱能突触囊泡标志物囊泡特异性蛋白聚糖的分布相当。当以合成VIP（猪序列）作为标准，通过放射免疫分析进行测量时，结扎电动神经会导致电叶（180%）和电动神经近端部分（130%）中VIP样免疫反应性显著积累，而电器官中的该反应性则下降（-50%）。电叶、电动神经和电器官提取物中的VIP样免疫反应性从半制备反相高效液相色谱柱上洗脱下来时呈现为单一峰，其保留时间与猪VIP的保留时间相似。在分析柱上以更高分辨率进行再色谱分析表明，从电叶和电器官中提取的VIP样免疫反应性的分子形式存在差异，这表明存在翻译后加工的可能性。