Division of Reproductive Medicine, Department of Obstetrics and Gynecology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
CMEx fertility center, Center of Medical Excellence, Chiang Mai University, Chiang Mai 50200, Thailand.
JBRA Assist Reprod. 2023 Mar 30;27(1):20-24. doi: 10.5935/1518-0557.20210118.
To determine whether human hydrosalpinx fluid might have a deleterious effect on the fertilization rate and embryonic development of the exposed mouse oocytes.
Mouse cumulus-oocyte complexes (COCs) were randomly allocated for exposure to pure hydrosalpinx fluid (100% HSF group, n=400), EBSS containing 50% of hydrosalpinx fluid (50% HSF group, n=320) and pure EBSS (control group, n=300).
The results showed that the fertilization rate in the 100% HSF group was significantly lower than the control group (64.0% versus 73.0%, p=0.031). The blastocyst formation rate was also lower in the 100% HSF group than 50% HSF and the control group (51.5% versus 56.9% versus 56.3%, respectively), but not statistically significant (p=0.275). There was no significant difference in the mean numbers of cells in the ICM, TE, and total cell number in blastocysts from the control group and two hydrosalpinx fluid exposure groups.
Human hydrosalpinx fluid has a negative effect on the fertilization rate of the exposed mouse oocytes. However, this effect was found only in undiluted concentration and does not affect the subsequence of embryonic development and blastocyst cell number.
确定人输卵管积水液是否对暴露的小鼠卵母细胞的受精率和胚胎发育有不良影响。
将小鼠卵丘-卵母细胞复合物（COCs）随机分配暴露于纯输卵管积水液（100%HSF 组，n=400）、含有 50%输卵管积水液的 EBSS（50%HSF 组，n=320）和纯 EBSS（对照组，n=300）中。
结果显示，100%HSF 组的受精率明显低于对照组（64.0%比 73.0%，p=0.031）。100%HSF 组的囊胚形成率也低于 50%HSF 组和对照组（51.5%比 56.9%比 56.3%，分别），但无统计学意义（p=0.275）。对照组和两个输卵管积水液暴露组的囊胚内细胞团（ICM）、滋养外胚层（TE）和总细胞数的平均细胞数无显著差异。
人输卵管积水液对暴露的小鼠卵母细胞的受精率有负面影响。然而，这种影响仅在未稀释浓度下发现，并不影响随后的胚胎发育和囊胚细胞数。
