Key Laboratory of Applied Chemistry, College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, 066004, China.
Key Laboratory of Applied Chemistry, College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, 066004, China.
Anal Biochem. 2022 Jun 1;646:114628. doi: 10.1016/j.ab.2022.114628. Epub 2022 Mar 4.
The stability of biosensors is of significant importance for practical applications, and the natural biomineralization processes in living organisms have inspired us from a new perspective. In this work, acetylcholinesterase (AChE) was embedded into zeolitic imidazolate framework-8 carriers (with negligible cytotoxicity) via biomimetic mineralization, being demonstrated to be a stable strategy for enzyme immobilization. When further coupled with the conductive and catalytic Au nanoparticles, the biocomposites were explored as electrochemical pesticide detection biosensor, which showed favorable analytical performance, and improved stability comparing with other biosensors. This work provides a new strategy for the reasonable design of stable biosensors for different analytes monitoring.
生物传感器的稳定性对于实际应用非常重要，而生物体中的自然生物矿化过程为我们提供了新的视角。在这项工作中，通过仿生矿化将乙酰胆碱酯酶（AChE）嵌入沸石咪唑骨架-8 载体（细胞毒性可忽略不计）中，证明这是一种稳定的酶固定化策略。当进一步与导电和催化的金纳米粒子结合时，将生物复合材料作为电化学农药检测生物传感器进行了探索，该传感器表现出良好的分析性能，并与其他生物传感器相比具有更好的稳定性。这项工作为针对不同分析物监测的稳定生物传感器的合理设计提供了新策略。
