Institute of Molecular Biology and Genetics, NAS of Ukraine, 150 Zabolotnogo str., 03143 Kyiv, Ukraine; IQVIA, 12 Amosova str., 03038 Kyiv, Ukraine.
Institute of Molecular Biology and Genetics, NAS of Ukraine, 150 Zabolotnogo str., 03143 Kyiv, Ukraine; Taras Shevchenko Kyiv National University, 64 Volodymyrska str., 01601 Kyiv, Ukraine.
Biomater Adv. 2022 Dec;143:213180. doi: 10.1016/j.bioadv.2022.213180. Epub 2022 Nov 3.
Biosensors are advanced devices for analysis of composition of blood, urine, environmental samples, and many other media. Their current development is tightly linked with nanomaterials, such as zeolites and zeolitic imidazolate framework (ZIFs). The present review describes electrochemical (amperometric, conductometric, ISFET) and optical (fluorescent and colorimetric) biosensors that incorporate zeolites and ZIFs in their biorecognition elements. The biosensors are based on immobilized enzymes (such as glucose oxidase, urease, and acetylcholinesterase), antibodies, DNA, and aptamers. The review present reasons for application of these nanomaterials, and discusses advantages of zeolite- and ZIF-containing biosensors over other biosensors. In most cases, the biosensors have improved sensitivity, better limit of detection, wider linear range, and other improved characteristics. It is demonstrated that immobilization of biomolecules such as enzymes or antibodies on the surface of zeolites and ZIFs enables creation of unique advanced biosensors that have a potential for further development and practical applications.
生物传感器是用于分析血液、尿液、环境样本和许多其他介质成分的先进设备。它们的当前发展与纳米材料（如沸石和沸石咪唑酯骨架（ZIFs））紧密相关。本综述描述了电化学（安培计、电导计、ISFET）和光学（荧光和比色）生物传感器，它们在生物识别元件中结合了沸石和 ZIFs。这些生物传感器基于固定化酶（如葡萄糖氧化酶、脲酶和乙酰胆碱酯酶）、抗体、DNA 和适体。本文介绍了应用这些纳米材料的原因，并讨论了含沸石和 ZIF 的生物传感器相对于其他生物传感器的优势。在大多数情况下，生物传感器具有更高的灵敏度、更好的检测限、更宽的线性范围和其他改进的特性。研究表明，将酶或抗体等生物分子固定在沸石和 ZIF 的表面上，可以制造出具有潜在进一步发展和实际应用的独特的先进生物传感器。
