Lane J M, Suda M, von der Mark K, Timpl R
J Orthop Res. 1986;4(3):318-29. doi: 10.1002/jor.1100040308.
A nonimmobilized rat tibial fracture model of endochondral osseous repair was examined for the unique localizations of specific collagen genetic types. At various stages of the healing process, the demineralized callus was reacted with immunofluorescent antibodies directed against the type specific forms of matrix collagen. Type III collagen rapidly appeared (day 8-10) and remained in the primitive mesenchymal callus until remodeled. It was particularly prominent in the highly vasoformative regions and the pericallus encapsulation but not present in preexisting cortical and neoformed lamellar bone. The type II collagen, a marker of cartilage, was uniquely located only in areas of chondroid differentiation and calcification. Type II collagen was absent from all bone and was not identified beneath the repairing intact periosteum. The differentiating chondrocytes synthesized type II collagen on an underlayer of type III collagen already within the mesenchymal matrix. From these studies of genetically unique collagen markers, it appears that only in areas of motion or anoxia does an intermediate of chondroid tissue appear. The utilization of specific type II and type III collagen immunofluorescent antibodies has facilitated the understanding of the fracture repair process and has acted as an indicator for unique matrix components.
对软骨内成骨修复的非固定大鼠胫骨骨折模型进行研究，以确定特定胶原基因类型的独特定位。在愈合过程的不同阶段，将脱钙骨痂与针对基质胶原特定类型形式的免疫荧光抗体反应。III型胶原迅速出现（第8 - 10天），并保留在原始间充质骨痂中直至重塑。它在高血管形成区域和骨痂周围包封中特别突出，但在原有的皮质骨和新形成的板层骨中不存在。II型胶原是软骨的标志物，仅独特地位于软骨样分化和钙化区域。所有骨中均不存在II型胶原，在修复完整的骨膜下也未发现。分化的软骨细胞在间充质基质内已有的III型胶原底层上合成II型胶原。从这些对基因独特的胶原标志物的研究来看，似乎只有在运动或缺氧区域才会出现软骨样组织中间体。特异性II型和III型胶原免疫荧光抗体的应用有助于理解骨折修复过程，并作为独特基质成分的指标。
