Department of Medical Microbiology, School of Laboratory Medicine and Medical Sciences, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa.
Department of Medical Biochemistry and Microbiology, Zoonosis Science Center, Uppsala University, Uppsala, Sweden; Department of Microbiology and Immunology, Faculty of Pharmacy, El Salehya El Gadida University, New Salhia City, Ash Sharqia, Egypt.
J Glob Antimicrob Resist. 2022 Jun;29:150-154. doi: 10.1016/j.jgar.2022.02.019. Epub 2022 Mar 5.
The resistome, virulome, mobilome and phylogenetic relationship of the Acinetobacter baumannii isolate FG121 depicting the multilocus sequence type (ST) 231 isolated from hospital effluent water in South Africa was determined using whole-genome sequence analysis.
A. baumannii FG121 was isolated on Leed Acinetobacter Medium (LAM) agar and the bacterial isolate was identified using the VITEK®2 platform. Antibiotic susceptibility testing was performed using Kirby-Bauer Disk diffusion method. A whole genome sequencing library was constructed from DNA extracted from the isolate using the Illumina Nextera XT library preparation kit and was sequenced using the Illumina NextSeq500 platform. Generated reads were de novo assembled using SpAdes v.3.9. The assembled contigs were annotated, and multilocus sequence type, antimicrobial resistance, and virulence genes were identified.
The resistome was consistent with the resistance phenotype of the isolate with resistance determinants for beta-lactams, aminoglycosides, and tetracycline (bla, bla, bla, bla, aph[3']-VIa and tet[B]). Global phylogenomic analysis using BacWGSTdb revealed that the isolate belonged to the multilocus sequence type ST-231, similar to previously reported isolates from South Africa, the United States, and related to the invasive KR3831 isolate identified from Oman in 2012, suggesting the isolate might be imported from abroad. Virulome analysis predicted both virulence and biofilm-determinants of A. baumannii, which may help to establish infections in adverse conditions.
This is the first report on a carbapenemase-encoding A. baumannii ST-231 isolated from hospital effluent water. Our data will offer insight into the global phylogenetic, pathogenicity and distribution of A. baumannii in South Africa.
通过全基因组序列分析，确定了南非医院污水中分离的多基因座序列型（ST）231 型鲍曼不动杆菌 FG121 的耐药组、毒力组、移动组和系统发育关系。
将 FG121 鲍曼不动杆菌分离到 Leed 不动杆菌培养基（LAM）琼脂上，并使用 VITEK®2 平台进行细菌鉴定。使用 Kirby-Bauer 圆盘扩散法进行抗生素敏感性测试。使用 Illumina Nextera XT 文库制备试剂盒从分离株中提取 DNA 构建全基因组测序文库，并使用 Illumina NextSeq500 平台进行测序。使用 SpAdes v.3.9 从头组装生成的读数。对组装的 contigs 进行注释，并鉴定多基因座序列型、抗微生物药物耐药性和毒力基因。
耐药组与分离株的耐药表型一致，具有β-内酰胺类、氨基糖苷类和四环素类（bla、bla、bla、bla、aph[3']-VIa 和 tet[B]）的耐药决定因素。使用 BacWGSTdb 进行的全球系统基因组分析表明，该分离株属于多基因座序列型 ST-231，与南非、美国的先前报告的分离株相似，并与 2012 年从阿曼鉴定的侵袭性 KR3831 分离株有关，这表明该分离株可能是从国外进口的。毒力组分析预测了鲍曼不动杆菌的毒力和生物膜决定因素，这可能有助于在不利条件下建立感染。
这是首次从医院污水中分离出碳青霉烯酶编码的鲍曼不动杆菌 ST-231 的报告。我们的数据将为了解南非鲍曼不动杆菌的全球系统发育、致病性和分布提供深入了解。
