Shi Yu-Jiao, Li Wen-Bao, Zhang Bo-Yao, Yao Guo-Wang, Shi Xiao-Hong
Key Laboratory of Water Resources Protection and Utilization, School of Water Conservancy and Civil Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China.
Huan Jing Ke Xue. 2022 Mar 8;43(3):1424-1433. doi: 10.13227/j.hjkx.202107108.
In order to study the bacterial community composition and corresponding function in Wuliangsu Lake at the end of the Hetao Plain during the irrigation gap period, lake samples were collected in September 2020, and the pH, TN, TP, DIP, DTP, NH-N, Chla, EC, SAL, and other indicators were analyzed. The 16S rRNA high-throughput sequencing method was used to explore the attached bacteria and bacterioplankton in 15 samples of the surface water in Wuliangsu Lake. The experimental results showed that:① the alpha diversity Chao and Shannon indices of attached bacteria were greater than that of bacterioplankton, but the median of Shannoneven index was the same. ② In each sampling point, the bacterioplankton of Proteobacteria and Actinobacteria in the top five dominant bacteria phyla were higher than that of attached bacteria, and the abundance of attached bacteria and bacterioplankton of Bacteroidota were staggered. On the contrary, the contents of attached bacteria of Verrucomicrobiota and Cyanobacteria were all higher than that of bacterioplankton. ③ Redundant analysis showed that pH had the most significant effect on dominant attached bacteria, and the effect of conductivity and salinity in dominant bacterioplankton was the most significant. ④ PICRUSt2 function prediction analysis showed that attached bacteria and planktonic bacteria had the strongest metabolic functions, showing abundant metabolic functions. There were 29 nitrogen-related effective KOs and 88 phosphorus-related effective KOs, with the greatest nitrogen-fixing function and strong inorganic phosphorus-dissolving function, and bacterioplankton played a greater role in the two functions.
为研究河套平原末端乌梁素海在灌溉间隙期的细菌群落组成及相应功能，于2020年9月采集湖泊样本，并对pH、TN、TP、DIP、DTP、NH-N、Chla、EC、SAL等指标进行分析。采用16S rRNA高通量测序方法探究乌梁素海15个地表水样本中的附着细菌和浮游细菌。实验结果表明：①附着细菌的α多样性Chao和Shannon指数大于浮游细菌，但Shannoneven指数中位数相同。②在各采样点，前五大优势菌门中变形菌门和放线菌门的浮游细菌高于附着细菌，拟杆菌门附着细菌和浮游细菌的丰度呈交错状态。相反，疣微菌门和蓝细菌附着细菌的含量均高于浮游细菌。③冗余分析表明，pH对优势附着细菌影响最显著，电导率和盐度对优势浮游细菌的影响最显著。④PICRUSt2功能预测分析表明，附着细菌和浮游细菌具有最强的代谢功能，呈现丰富的代谢功能。有29个与氮相关的有效KO和88个与磷相关的有效KO，固氮功能最强，无机磷溶解功能较强，浮游细菌在这两种功能中发挥的作用更大。
